caa a22 4500 463235878 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9188-x doi (NATIONALLICENCE)springer-10.1007/s11240-006-9188-x Debnath Samir Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, 308 Brookfield Road, P.O. 39088, A1E 5Y7, St. John's, Newfoundland and Labrador, Canada aut A two-step procedure for in vitro multiplication of cloudberry ( Rubus chamaemorus L.) shoots using bioreactor [Elektronische Daten] [Samir Debnath] Cultures of three cloudberry (Rubus chamaemorus L.) clones collected from natural stands in Newfoundland and Labrador, Canada were established in vitro on a modified cranberry (Vaccinium macrocarpon Ait.) tissue culture medium containing 8.9μM 6-benzylaminopurine (BAP). Clones were compared for in vitro shoot proliferation on gelled medium supplemented with varying levels of BAP and thidiazuron (TDZ). Addition of 5.8μM gibberellic acid (GA3) in 8.9μM BAP-contained medium improved shoot proliferation. TDZ supported rapid shoot proliferation at low concentration (1.1μM) but induced 20-30% hyperhydricity in a plastic airlift bioreactor system containing liquid medium. Bioreactor-multiplied hyperhydric shoots were transferred to gelled medium containing 8.9μM BAP and 5.8μM GA3 and produced normal shoots within 4 weeks of culture. Genotypes differed significantly with respect to multiplication rate with ‘C1' producing the most shoots per explant. Proliferated shoots were rooted on a potting medium with 65-75% of survivability of rooted plants. Present results suggested the possibility of large-scale multiplication of cloudberry shoots in bioreactors. Springer Science+Business Media B.V., 2006 Airlift bioreactor nationallicence Liquid culture nationallicence Micropropagation nationallicence Cytokinin nationallicence Wild clones nationallicence BAP : 6-Benzylaminopurine nationallicence IBA : Indole-3-butyric acid nationallicence GA3 : Gibberellic acid nationallicence PPF : Photosynthetic photon flux nationallicence TDZ : Thidiazuron nationallicence Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 185-191 0167-6857 88:2<185 2007 88 11240 https://doi.org/10.1007/s11240-006-9188-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9188-x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Debnath Samir Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, 308 Brookfield Road, P.O. 39088, A1E 5Y7, St. John's, Newfoundland and Labrador, Canada aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 185-191 0167-6857 88:2<185 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer