caa a22 4500 463235886 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9193-0 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9193-0 Patterns of storage protein and triacylglycerol accumulation during loblolly pine somatic embryo maturation [Elektronische Daten] [Disa Brownfield, Christopher Todd, Sandra Stone, Michael Deyholos, David Gifford] Conifer somatic embryo germination and early seedling growth are fundamentally different than in their zygotic counterparts in that the living maternal megagametophyte tissue surrounding the embryo is absent. The megagametophyte contains the majority of the seed storage reserves in loblolly pine and the lack of the megagametophyte tissue poses a significant challenge to somatic embryo germination and growth. We investigated the differences in seed storage reserves between loblolly pine mature zygotic embryos and somatic embryos that were capable of germination and early seedling growth. Somatic embryos utilized in this study contained significantly lower levels of triacylglycerol and higher levels of storage proteins relative to zygotic embryos. A shift in the ratio of soluble to insoluble protein present was also observed. Mature zygotic embryos had roughly a 3:2 ratio of soluble to insoluble protein whereas the somatic embryos contained over 5-fold more soluble protein compared to insoluble protein. This indicates that the somatic embryos are not only producing more protein overall, but that this protein is biased more heavily towards soluble protein, indicating possible differences in metabolic activity at the time of desiccation. Springer Science+Business Media B.V., 2007 Embryogenesis nationallicence Pinus taeda nationallicence Seed storage reserves nationallicence Lipids nationallicence Zygotic embryo nationallicence ABA : Abscisic acid nationallicence PEG : Polyethylene glycol nationallicence TAG : Triacylglycerol nationallicence Brownfield Disa Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut Todd Christopher Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut Stone Sandra Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut Deyholos Michael Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut Gifford David Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 217-223 0167-6857 88:2<217 2007 88 11240 https://doi.org/10.1007/s11240-006-9193-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9193-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brownfield Disa Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut NATIONALLICENCE 700 1- Todd Christopher Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut NATIONALLICENCE 700 1- Stone Sandra Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut NATIONALLICENCE 700 1- Deyholos Michael Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut NATIONALLICENCE 700 1- Gifford David Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 217-223 0167-6857 88:2<217 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer