caa a22 4500 463235908 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9169-0 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9169-0 The role of BAP in somatic embryogenesis induction from seed explants of Arachis species from Sections Erectoides and Procumbentes [Elektronische Daten] [G. Pacheco, R. Gagliardi, L. Carneiro, C. Callado, J. Valls, E. Mansur] Somatic embryogenesis was induced from seed explants of Arachis archeri, A. porphyrocalix (Section Erectoides) and A. appressipila (Section Procumbentes) in response to 6-benzylaminopurine (BAP). Embryo axes first developed into single shoots in response to 4.4μM BAP. Friable embryogenic calluses were produced from the hypocotyl region of these explants in response to different BAP concentrations. Embryonic leaflets also gave rise to friable calluses, but somatic embryos were only observed in explants of A. archeri and A. appressipila. Histological analyses revealed the presence of heart-shaped, torpedo and cotyledonary stages embryos, both as isolated and fused structures. A low frequency of embryo-to-plant conversion was achieved by inducing shoot development on medium solidified with 0.5% phytagel and supplemented with 1.5% or 3% sucrose. Rooting was induced on MS supplemented with indole-3-acetic acid (IAA). Springer Science+Business Media B.V., 2006 Arachis nationallicence Groundnut nationallicence Histology nationallicence Micropropagation nationallicence Somatic embryogenesis nationallicence BAP : 6-Benzylaminopurine nationallicence IAA : Indole-3-acetic acid nationallicence Pacheco G. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut Gagliardi R. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut Carneiro L. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut Callado C. Departamento de Botânica, UERJ, Rio de Janeiro, Brazil aut Valls J. Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil aut Mansur E. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 121-126 0167-6857 88:2<121 2007 88 11240 https://doi.org/10.1007/s11240-006-9169-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9169-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pacheco G. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Gagliardi R. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Carneiro L. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Callado C. Departamento de Botânica, UERJ, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Valls J. Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil aut NATIONALLICENCE 700 1- Mansur E. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 121-126 0167-6857 88:2<121 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer