caa a22 4500 463235924 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9255-y doi (NATIONALLICENCE)springer-10.1007/s11240-007-9255-y Comparison of callus induction and plant regeneration from different explants in triploid and tetraploid turf-type bermudagrasses [Elektronische Daten] [Song Zhang, Wayne Hanna, Peggy Ozias-Akins] The turf-type bermudagrasses are genetically variable and do not respond uniformly to tissue culture and plant regeneration protocols. We evaluated the callus induction response of two explant types, young inflorescences and nodes, from multiple genotypes including triploid TifSport, TifEagle, and Tift97-4 and tetraploid Tift93-132, Tift93-135, Tift93-156 and Tift93-157 on MS medium supplemented with 1-1.5mgl−1 2,4-D+0.01mgl−1 BA+1.16gl−1 proline. Four types of callus were observed. Type I was fluffy, soft, and white non-embryogenic callus, common to all cultures. Type II was globular, transparent, and hard, but sticky callus, which was pre-embryogenic and could be selected for subculture. Type III callus was transparent, compact, and embryogenic. Type IV callus was opaque white and compact. Both Type III and Type IV calluses were embryogenic and regenerative. A combination of gelling agents in the medium (2gl−1 Gelrite and 5gl−1 agar) improved callus quality and increased the rate of compact callus formation during subculture. Embryogenesis from compact callus was observed in TifEagle, TifSport and Tift93-132, and shoots were regenerated on MS medium with 0.1mgl−1 2,4-D+0.5-4.0mgl−1 BA. Low intensity light treatment (30μmolm−2s−1 of cool white fluorescence) to callus before regeneration greatly enhanced regeneration frequency from 6.7% to 40% in recalcitrant TifSport. Springer Science+Business Media B.V., 2007 Bermudagrass nationallicence Callus induction and subculture nationallicence Embryogenic callus nationallicence Regeneration nationallicence 2,4- D : 2,4-Dichlorophenoxyacetic acid nationallicence BA : 6-Benzyladenine nationallicence MS : Murashige and Skoog nationallicence NAA : α-Naphthaleneacetic acid nationallicence TDZ : Thidiazuron nationallicence GA3 : Gibberellic acid nationallicence ABA : Abscisic acid nationallicence Zhang Song Department of Crop and Soil Science, University of Georgia, Tifton Campus, 31794, Tifton, GA, USA aut Hanna Wayne Department of Crop and Soil Science, University of Georgia, Tifton Campus, 31794, Tifton, GA, USA aut Ozias-Akins Peggy Department of Horticulture, University of Georgia, Tifton Campus, 31794, Tifton, GA, USA aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 71-78 0167-6857 90:1<71 2007 90 11240 https://doi.org/10.1007/s11240-007-9255-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9255-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Song Department of Crop and Soil Science, University of Georgia, Tifton Campus, 31794, Tifton, GA, USA aut NATIONALLICENCE 700 1- Hanna Wayne Department of Crop and Soil Science, University of Georgia, Tifton Campus, 31794, Tifton, GA, USA aut NATIONALLICENCE 700 1- Ozias-Akins Peggy Department of Horticulture, University of Georgia, Tifton Campus, 31794, Tifton, GA, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 71-78 0167-6857 90:1<71 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer