caa a22 4500 463235932 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9259-7 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9259-7 Polyamine and nitric oxide levels relate with morphogenetic evolution in somatic embryogenesis of Ocotea catharinensis [Elektronische Daten] [Claudete Santa-Catarina, Vanildo Silveira, Günther Scherer, Eny Floh] In this study we examined the effect of polyamines (PAs) putrescine (Put), spermidine (Spd) and spermine (Spm) on growth, morphology evolution, endogenous PAs levels and nitric oxide (NO) release in Ocotea catharinensis somatic embryo cultures. We observed that Spd and Spm reduced culture growth, permitted embryo morphogenetic evolution from the earliest to last embryo development stages, increased endogenous PAs levels, and induced NO release in O. catharinensis somatic embryos. On the other hand, Put had little effect on these parameters. Spd and Spm could successfully be used to promote somatic embryo maturation in O. catharinensis. The results suggest that Spd and Spm have an important role during the growth, development and morphogenetic evolution of somatic embryos, through alterations in the endogenous nitric oxide and PAs metabolism in this species. Springer Science+Business Media B.V., 2007 Somatic embryo development nationallicence Maturation nationallicence Putrescine nationallicence Spermidine nationallicence Spermine nationallicence NO : Nitric oxide nationallicence PAs : Polyamines nationallicence Put : Putrescine nationallicence Spd : Spermidine nationallicence Spm : Spermine nationallicence WPM : Wood plant medium nationallicence Santa-Catarina Claudete Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut Silveira Vanildo Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut Scherer Günther Universität Hannover, Institut für Zierpflanzenbau, AG Spezielle Ertragsphysiologie, Herrenhäuser Str. 2, 30419, Hannover, Germany aut Floh Eny Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 93-101 0167-6857 90:1<93 2007 90 11240 https://doi.org/10.1007/s11240-007-9259-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9259-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santa-Catarina Claudete Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Silveira Vanildo Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Scherer Günther Universität Hannover, Institut für Zierpflanzenbau, AG Spezielle Ertragsphysiologie, Herrenhäuser Str. 2, 30419, Hannover, Germany aut NATIONALLICENCE 700 1- Floh Eny Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 93-101 0167-6857 90:1<93 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer