caa a22 4500 463235940 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9247-y doi (NATIONALLICENCE)springer-10.1007/s11240-007-9247-y Somatic embryogenesis in strawberry ( Fragaria sp.) through callus culture [Elektronische Daten] [Manosh Biswas, Rafiul Islam, Monzur Hossain] Somatic embryogenesis was achieved from callus from leaf and nodal segments of strawberry clone pbgel-2000. The highest percent of cultures with somatic embryos was achieved on MS medium supplemented with 1.0 mg/l 2,4-D, 0.5 mg/l BAP and 50% proline. Darkness was the best condition for incubation with daily light periods over 6h reducing the frequency of embryogenesis. Regenerated plants (90-95%) were successfully transferred to soil, showed normal morphology and produced fruit four months after planting. Springer Science+Business Media B.V., 2007 2,4-D nationallicence BAP nationallicence Internodes nationallicence Leaf nationallicence NAA nationallicence Organogenesis nationallicence 2, 4-D : 2, 4 dichlorophenoxyacetic acid nationallicence BAP : 6 benzylaminopurine nationallicence CV : Coefficient of variability nationallicence DMRT : Duncan's multiple range tests nationallicence GA3 : Gibberellic acid nationallicence IBA : Indole-3-butyric acid nationallicence MS : Murashige and Skoog basal medium nationallicence NAA : α-naphthaleneacetic acid nationallicence PIPES : Piperazine-N, N-(2-ethanesulfonic acid) nationallicence SE : Standard error nationallicence Biswas Manosh Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh aut Islam Rafiul Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh aut Hossain Monzur Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 49-54 0167-6857 90:1<49 2007 90 11240 https://doi.org/10.1007/s11240-007-9247-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9247-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Biswas Manosh Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh aut NATIONALLICENCE 700 1- Islam Rafiul Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh aut NATIONALLICENCE 700 1- Hossain Monzur Department of Botany, University of Rajshahi, 6205, Rajshahi, Bangladesh aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 49-54 0167-6857 90:1<49 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer