caa a22 4500 463235959 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9233-4 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9233-4 Optimizing the micropropagation protocol for the endangered Aloe polyphylla [Elektronische Daten] can meta -topolin and its derivatives serve as replacement for benzyladenine and zeatin? [Michael Bairu, Wendy Stirk, Karel Dolezal, Johannes Van Staden] Benzyladenine (BA) is the most widely used cytokinin in the micropropagation industry due to its effectiveness and affordability. It, however, has disadvantages such as genetic alteration and abnormal growth in some plants. Naturally occurring zeatin on the other hand is not as widely used as BA and is far more expensive. The use of meta-topolin and its derivatives as alternatives to BA and zeatin, both of which frequently have negative effects in tissue culture was investigated. In vitro grown Aloe polyphylla (an endangered medicinal and ornamental aloe) were cultured on full strength Murashige and Skoog basal medium with different concentrations of cytokinins and solidified with 1% Bacteriological Agar (Oxoid No. 1). mT was the preferred cytokinin both in terms of multiplication rate and rooting. The optimum concentration that induced regeneration and rooting is 5.0μM. The problem of hyperhydricity was totally controlled. Plants rooted spontaneously in multiplication medium, thus avoiding the extra rooting step of the protocol. More than 91% of the plants transferred to ex vitro conditions were successfully acclimatized. Springer Science+Business Media B.V., 2007 Aromatic cytokinins nationallicence Conservation nationallicence Hyperhydricity nationallicence Metabolites nationallicence Multiplication rate nationallicence Plant growth regulators nationallicence Root and shoot growth nationallicence [9G]BA : [6-benzylamino-9- β-d-glucopyranosylpurine] nationallicence BA : [6-benzylaminopurine] nationallicence IBA : indole butyric acid nationallicence MS : Murashige and Skoog (1962) medium nationallicence Me m T : [6-(3-methoxybenzylamino)purine] nationallicence Me m TR : [6-(3-methoxybenzylamino)-9-β-d-ribofuranosylpurine] nationallicence m T : [6-(3-hydroxybenzylamino)purine] nationallicence m TR : [6-(3-hydroxybenzylamino)-9-β-d-ribofuranosylpurine] nationallicence o T : [6-(2-hydroxybenzylamino)purine] nationallicence PGR : plant growth regulators nationallicence p T : [6-(4-hydroxybenzylamino)purine] nationallicence Bairu Michael Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Stirk Wendy Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Dolezal Karel Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany AS CR, 783 71, Olomouc-Holice, Czech Republic aut Van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 15-23 0167-6857 90:1<15 2007 90 11240 https://doi.org/10.1007/s11240-007-9233-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9233-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bairu Michael Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Stirk Wendy Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Dolezal Karel Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany AS CR, 783 71, Olomouc-Holice, Czech Republic aut NATIONALLICENCE 700 1- Van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 15-23 0167-6857 90:1<15 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer