caa a22 4500 463235975 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9251-2 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9251-2 Invitro morphogenesis of sunflower ( Helianthus annuus ) hypocotyl protoplasts: the effects of protoplast density, haemoglobin and spermidine [Elektronische Daten] [Elena Rákosy-Tican, Adriana Aurori, Simona Vesa, Krisztina-Maria Kovacs] Sunflower, as one of the most important oil-producing crops, represents an important target for genetic improvement through gene transfer or somatic hybridization. Unfortunately, sunflower is recognized as recalcitrant to in vitro culture. The aim of our paper was to improve sunflower protoplast regeneration. Three cultivars (Romanian hybrids) and one inbred line were used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in alginate disks and cultured in two plating densities, using two culture regimes as indicated by previous authors. Plating efficiency, callus development and plant regeneration were evaluated as well as old callus histology. In cv. ‘Select', the effects of 1:50 haemoglobin and 1mM spermidine were assayed on asymmetric division and/or plating efficiency. Plant regeneration from hypocotyl protoplasts was achieved for two cvs., ‘Florom 328' and ‘Turbo', with the former proving once more its totipotency. The best culture regime proved to be as recommended by Krasnyanski and Menczel (1993), but the best density in the culture medium was the highest ever tested, 8×105ppml−1. Moreover, the histology of old green compact protoplast-derived callus revealed a very well organized structure suggesting senescence. In the non-responsive cv. ‘Select', haemoglobin was found to stimulate protoplast asymmetric division and the development of heart-shaped embryo-like structures, while spermidine stimulated overall protoplast plating efficiency. Springer Science+Business Media B.V., 2007 Alginate disk nationallicence Embryogenesis nationallicence Embryo-like structures nationallicence Inbreed line nationallicence Hybrids nationallicence Old callus histology nationallicence Organogenesis nationallicence Oxygenation nationallicence Protoplast immobilization nationallicence Protoplast plating efficiency nationallicence Cv. : Cultivar nationallicence GA3 : Gibberellic acid nationallicence MS : Murashige and Skoog (1962) nationallicence Ppml−1 : Protoplasts ml−1 nationallicence Hae : Haemoglobin nationallicence SP : Spermidine nationallicence Rákosy-Tican Elena Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut Aurori Adriana Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut Vesa Simona Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut Kovacs Krisztina-Maria Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 55-62 0167-6857 90:1<55 2007 90 11240 https://doi.org/10.1007/s11240-007-9251-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9251-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rákosy-Tican Elena Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Aurori Adriana Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Vesa Simona Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Kovacs Krisztina-Maria Faculty of Biology and Geology, Department of Experimental Biology, Plant Genetic Engineering Group, "Babeş-Bolyai” University, Clinicilor str. 5-7, 400006, Cluj-Napoca, Romania aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 55-62 0167-6857 90:1<55 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer