caa a22 4500 463235983 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9256-x doi (NATIONALLICENCE)springer-10.1007/s11240-007-9256-x Somatic embryogenesis from whole flowers, anthers and ovaries of grapevine ( Vitis spp.) [Elektronische Daten] [Giorgio Gambino, Paola Ruffa, Rosalina Vallania, Ivana Gribaudo] A novel method for initiating somatic embryogenesis in grapevine, based on immature whole flower culture, is presented. The embryogenic competence of flowers was compared to that of anthers and ovaries, the most widely used explant types, for five grapevine cultivars. Both the genotype and the explant source affected the differentiation of somatic embryos. The highest percentages of embryogenesis were obtained in ovary-derived calli from all cultivars tested with the exception of Brachetto a grappolo lungo. Whole flowers proved to be suitable material for initiating embryogenic cultures for most tested cultivars, and for 110 R, Chardonnay, and Grignolino they gave similar or better results than anthers. Collection of whole flowers from the inflorescence is easier and faster than excision of anthers and ovaries from the flower itself; it can be done without the use of a stereomicroscope and damage to the explant is unlikely. No morphological difference was noted among embryogenic cultures originated from ovaries, flowers, or anthers. Springer Science+Business Media B.V., 2007 Embryogenic callus nationallicence Floral explants nationallicence Genotype effect nationallicence Regeneration nationallicence Vitis nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence BA : 6-Benzyladenine nationallicence NOA : 2-Napthoxyacetic acid nationallicence IAA : Indole-3-acetic acid nationallicence Gambino Giorgio Plant Virology Institute CNR, Grugliasco Unit, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut Ruffa Paola Fondazione Giovanni Dalmasso, c/o Department of Arboriculture and Pomology, University of Torino, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut Vallania Rosalina Plant Virology Institute CNR, Grugliasco Unit, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut Gribaudo Ivana Plant Virology Institute CNR, Grugliasco Unit, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 79-83 0167-6857 90:1<79 2007 90 11240 https://doi.org/10.1007/s11240-007-9256-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9256-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gambino Giorgio Plant Virology Institute CNR, Grugliasco Unit, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Ruffa Paola Fondazione Giovanni Dalmasso, c/o Department of Arboriculture and Pomology, University of Torino, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Vallania Rosalina Plant Virology Institute CNR, Grugliasco Unit, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Gribaudo Ivana Plant Virology Institute CNR, Grugliasco Unit, Via L. da Vinci 44, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 79-83 0167-6857 90:1<79 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer