caa a22 4500 463235991 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9232-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9232-5 Mother plant age and seasonal influence on invitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece [Elektronische Daten] [Epaminondas Kartsonas, Maria Papafotiou] A micropropagation method for Quercus euboica Pap. was developed. Nodal explants from seedlings gave higher multiplication rates than explants from adult plants. Cultures initiated at the beginning of May produced the highest percentage of shoot forming explants and multiplication rate. Woody Plant Medium (WPM) salts, with 100mgl−1 myoinositol, 1mgl−1 thiamine, 0.5mgl−1 pyridoxine, 0.5mgl−1 nicotinic acid and 3% sucrose was used as basal medium and several cytokinins at various concentrations were evaluated for their effect on shoot multiplication. The highest shoot multiplication rate was obtained with 4.44μΜ BA. IBA at 9.84μΜ in the culture medium during the first week of culture, and if followed by culture in hormone-free medium, gave the best rooting results. Darkness at the beginning of the rooting period did not improve rooting. The use of plastic wrap as a cover material of the culture vessels enhanced rooting percentage and root number. Plantlets acclimatized exvitro in soil from the natural environment of the species survived at a higher percentage (up to 93%) and had more vigorous growth than plantlets grown in a compost-perlite (2:1 v/v) medium (up to 36%). Springer Science+Business Media B.V., 2007 Acclimatization nationallicence Conservation biotechnology nationallicence Cytokinins nationallicence Invitro rooting nationallicence Micropropagation nationallicence Proliferation nationallicence Shoot multiplication nationallicence BA : 6-Benzyladenine nationallicence IBA : Indole-3-butyric acid nationallicence 2iP : N 6-(2-isopentenyl)adenine nationallicence NAA : 1-Naphteleneacetic acid nationallicence TDZ : Thidiazuron nationallicence WPM : Woody plant medium nationallicence Kartsonas Epaminondas Laboratory of Floriculture and Landscape Architecture, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece aut Papafotiou Maria Laboratory of Floriculture and Landscape Architecture, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 111-116 0167-6857 90:1<111 2007 90 11240 https://doi.org/10.1007/s11240-007-9232-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9232-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kartsonas Epaminondas Laboratory of Floriculture and Landscape Architecture, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece aut NATIONALLICENCE 700 1- Papafotiou Maria Laboratory of Floriculture and Landscape Architecture, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 111-116 0167-6857 90:1<111 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer