caa a22 4500 463236009 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9260-1 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9260-1 Genetic transformation of Torenia fournieri using the PMI/mannose selection system [Elektronische Daten] [Hong-Qing Li, Pei-Jing Kang, Mei-Lan Li, Mei-Ru Li] Transgenic torenia plants were obtained using the selectable marker gene phosphomannose isomerase (manA), which encodes the enzyme phosphomannose isomerase (PMI) to enable selection of transformed cells on media containing mannose. We found that shoot organogenesis in torenia leaf explants was effectively suppressed on medium supplemented with mannose, which indicated that torenia cells had little or no PMI activity and could not utilize mannose as a carbon source. Leaf pieces from invitro-germinated plants were inoculated with Agrobacterium tumefaciens EHA105 containing the binary vector pKPJ with both hpt and ManA genes, and subsequently selected on shoot induction (SI) medium (half strength MS basal+4.4μM BA+0.5μM NAA) supplemented with 20gl−1 mannose and 5gl−1 sucrose as carbon sources. Transformed plants were confirmed by PCR and Southern blot. The transgene expression was evaluated using Northern blot and the chlorophenol red assay. The transformation efficiency ranged from 7% to 10%, which is 1-3% higher than that obtained by selection with hygromycin. This system provides an efficient manner for selecting transgenic flower plants without using antibiotics or herbicides. Springer Science+Business Media B.V., 2007 Agrobacterium -mediated transformation nationallicence Mannose selection nationallicence Phosphomannose isomerase nationallicence Transgenic torenia nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence BA : Benzyladenine nationallicence IBA : Indole-3-butyric acid nationallicence MS : Murashige and Skoog's salts nationallicence NAA : α-Naphthaleneacetic acid nationallicence PMI : Phosphomannose isomerase nationallicence AS : Acetosyringone nationallicence Li Hong-Qing Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, 510631, Guangzhou, P.R. China aut Kang Pei-Jing Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, 510631, Guangzhou, P.R. China aut Li Mei-Lan College of Horticulture, Shanxi Agricultural University, 030801, Taigu, P.R. China aut Li Mei-Ru South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, P.R. China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 103-109 0167-6857 90:1<103 2007 90 11240 https://doi.org/10.1007/s11240-007-9260-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9260-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Hong-Qing Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, 510631, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Kang Pei-Jing Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, 510631, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Li Mei-Lan College of Horticulture, Shanxi Agricultural University, 030801, Taigu, P.R. China aut NATIONALLICENCE 700 1- Li Mei-Ru South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, P.R. China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 103-109 0167-6857 90:1<103 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer