caa a22 4500 463236017 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9246-z doi (NATIONALLICENCE)springer-10.1007/s11240-007-9246-z Overexpression of the Na+/H+ antiporter gene from Suaeda salsa confers cold and salt tolerance to transgenic Arabidopsis thaliana [Elektronische Daten] [Jinyao Li, Gangqiang Jiang, Ping Huang, Ji Ma, Fuchun Zhang] Na+/H+ exchanger catalyzes the countertransport of Na+ and H+ across membranes. By using reverse transcription PCR (RT-PCR) technique and Rapid Amplification of cDNA Ends (RACE), a Suaeda salsa cDNA clone was isolated and the sequence contains 2133bp with an open reading frame of 1674bp. The deduced amino acid sequence is similar to that of AtNHX1 and NHE isoforms in mammals, and shares high similarity with the sequences within predicted transmembrane segments and an amiloride-binding domain. In an attempt to improve salt tolerance of Arabidopsis plants, an expression vector containing SsNHX1 cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into Arabidopsis plants. Transgenic plants could normally grow and complete life cycle under treatment with 200mM NaCl but the wild-type Arabidopsis plant growth were severely retarded. Transgenic seedlings could resume and keep normal growth after treatment at −7°C for 5h but wt plants became hoariness and then all died. Springer Science+Business Media B.V., 2007 Cold tolerance nationallicence Na+/H+ exchanger nationallicence Salt tolerance nationallicence SsNHX1 nationallicence Suaeda salsa nationallicence NHX1 : Vacuolar Na+/H+ Exchanger nationallicence RACE : Rapid Amplification of cDNA Ends nationallicence RT-PCR : Reverse transriptase polymerase chain reaction nationallicence WT : Wildtype nationallicence Li Jinyao Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut Jiang Gangqiang Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut Huang Ping Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut Ma Ji Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut Zhang Fuchun Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 41-48 0167-6857 90:1<41 2007 90 11240 https://doi.org/10.1007/s11240-007-9246-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9246-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jinyao Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut NATIONALLICENCE 700 1- Jiang Gangqiang Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut NATIONALLICENCE 700 1- Huang Ping Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut NATIONALLICENCE 700 1- Ma Ji Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut NATIONALLICENCE 700 1- Zhang Fuchun Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, P. R. China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 41-48 0167-6857 90:1<41 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer