caa a22 4500 463236025 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9253-0 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9253-0 Chromatin reorganization and endogenous auxin/cytokinin dynamic activity during somatic embryogenesis of cultured cotton cell [Elektronische Daten] [Fanchang Zeng, Xianlong Zhang, Shuangxia Jin, Lei Cheng, Shaoguang Liang, Lisong Hu, Xiaoping Guo, Yichun Nie, Jinglin Cao] We conducted a systematic assessment and comparative study on the biochemical and cellular characteristics of cultured cotton cells during the entire process of somatic embryogenesis (SE). All staged cultures were widely investigated in this assay. Cell and tissue ectogenesis manipulation combined with flow cytometry (FCM) was employed to cellular study during the whole totipotency process of dedifferentiation and redifferentiation. We identified two phases of chromatin decondensation during the dedifferentiation and redifferentiation. At the same time, sharp increase in the ratio of indoleacetic acid (IAA), isopentenyladenosine group (iPAs) at the same stage of cell dedifferentiation and redifferentiation process serve as distinct biochemical maker of dedifferentiation and SE initiation with the unique feature. Our results suggest the two phases of chromatin reorganization associated with endogenous auxin/cytokinin dynamic activity may underlie dedifferentiation and redifferentiation during the entire SE process in cotton. Springer Science+Business Media B.V., 2007 Chromatin reorganization nationallicence Dynamic activity nationallicence Endogenous auxin/cytokinin nationallicence Flow cytometry nationallicence Gossypium hirsutum nationallicence Somatic embryogenesis nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence EC : Embryogenic calli nationallicence FCM : Flow cytometry nationallicence MS : Murashige and Skoog medium nationallicence NEC : Nonembryogenic calli nationallicence PEM : Proembryogenic masses nationallicence SE : Somatic embryogenesis nationallicence IAA : Indoleacetic acid nationallicence iPAs : Isopentenyladenosine group nationallicence Zeng Fanchang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Zhang Xianlong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Jin Shuangxia National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Cheng Lei National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Liang Shaoguang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Hu Lisong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Guo Xiaoping National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Nie Yichun National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Cao Jinglin National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 63-70 0167-6857 90:1<63 2007 90 11240 https://doi.org/10.1007/s11240-007-9253-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9253-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng Fanchang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Zhang Xianlong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Jin Shuangxia National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Cheng Lei National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Liang Shaoguang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Hu Lisong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Guo Xiaoping National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Nie Yichun National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Cao Jinglin National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 63-70 0167-6857 90:1<63 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer