caa a22 4500 46323605X CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11240-007-9258-8 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9258-8 Zeolin is a recombinant storage protein that can be used to produce value-added proteins in alfalfa ( Medicago sativa L.) [Elektronische Daten] [Michele Bellucci, Francesca De Marchis, Sergio Arcioni] The specific features of plants make them particularly suitable for the production of recombinant proteins and alfalfa is one of the recommended plant production systems. We have transformed alfalfa with a gene coding for a chimaeric protein made previously by fusing phaseolin to the N-terminal region of γ-zein and have analyzed the accumulation of this fusion protein, named zeolin. Zeolin was expressed both in T0 Regen SY alfalfa plants and in the progeny resulting from the sexual cross between Regen SY transformants and alfalfa cv. Adriana plants. In some alfalfa plants a 95kDa zeolin glycosylated polypeptide is the most abundant polypeptide detected by Western-blot analysis, whereas in tobacco the most abundant zeolin polypeptide has a molecular mass around 60kDa, expected for intact zeolin. Zeolin has been stably accumulated in alfalfa leaves because it forms endoplasmic reticulum-located protein bodies in the cell. As regards zeolin quantisation, in the progeny alfalfa plants a value of about 0.22-0.28mg of zeolin / g of fresh leaf weight has been estimated. Springer Science+Business Media B.V., 2007 Nuclear transformation nationallicence Fusion protein nationallicence Phaseolin nationallicence Zein nationallicence Bellucci Michele Institute of Plant Genetics—Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy aut De Marchis Francesca Institute of Plant Genetics—Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy aut Arcioni Sergio Institute of Plant Genetics—Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 85-91 0167-6857 90:1<85 2007 90 11240 https://doi.org/10.1007/s11240-007-9258-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9258-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bellucci Michele Institute of Plant Genetics—Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy aut NATIONALLICENCE 700 1- De Marchis Francesca Institute of Plant Genetics—Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy aut NATIONALLICENCE 700 1- Arcioni Sergio Institute of Plant Genetics—Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/1(2007-07-01), 85-91 0167-6857 90:1<85 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer