caa a22 4500 463236084 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9266-8 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9266-8 Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars [Elektronische Daten] [Xiao-Dong Cai, Jing Fu, Xiu-Xin Deng, Wen-Wu Guo] Cytoplasmic male sterility (CMS) is known to be controlled by mitochondrial genome in higher plants including Satsuma mandarin (Citrus unshiu Marc.). Citrus symmetric fusion experiments often produce diploid cybrids possessing nuclear DNA from the mesophyll parent and mitochondrial DNA (mtDNA) from the embryogenic callus parent. Therefore, it is possible to transfer CMS from Satsuma mandarin as callus parent to seedy citrus cultivars as leaf one by somatic cybridization. Herein, symmetric fusion technique was adopted to create cybrids for potential seedlessness by transferring CMS from Citrus unshiu Marc. cv. Guoqing No. 1 (G1) to two traditional Chinese seedy citrus cultivars, ‘Shatian' pummelo (C. grandis (L) Osbeck) and ‘Bingtang' orange (C. sinensis (L) Osbeck). Flow cytometry analysis showed that 19 plants recovered from G1+‘Bingtang' orange and 17 of 35 plants regenerated from G1+‘Shatian' pummelo were diploid. The remaining plants from G1+‘Shatian' pummelo were tetraploid. The diploid plants from the two combinations were confirmed as true cybrids by simple sequence repeat (SSR) and cleaved amplified polymorphic sequence (CAPS) analysis, with nuclear DNA from their corresponding leaf parent and mtDNA from their common suspension parent, G1 Satsuma mandarin. The remaining plants from G1+‘Shatian' pummelo were identified as somatic hybrids with mtDNA from G1. The chloroplast simple sequence repeat (cp-SSR) analysis revealed somatic hybrid/cybrid plants from the two combinations in most cases possessed either of their parental chloroplast type, and two plants from G1+‘Shatian' pummelo and all embryoids analyzed from G1+‘Bingtang' orange possessed chloroplast DNA (cpDNA) from both parents. These results demonstrated that we succeeded in introducing mtDNA from G1 Satsuma mandarin into the two target seedy citrus cultivars for potential seedlessness through symmetric fusion. Springer Science+Business Media B.V., 2007 Citrus nationallicence Cybrids nationallicence Cytoplasmic male sterility nationallicence Molecular analysis nationallicence Seedlessness nationallicence Somatic hybridization nationallicence CAPS : Cleaved amplified polymorphic sequence nationallicence CpDNA : Chloroplast DNA nationallicence cp-SSR : Chloroplast simple sequence repeat nationallicence G1 : Satsuma mandarin, Citrus unshiu Marc. cv. Guoqing No. 1 nationallicence SSR : Simple sequence repeat nationallicence MtDNA : Mitochondrial DNA nationallicence MT : Murashige and Tucker (1969) nationallicence Cai Xiao-Dong National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut Fu Jing National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut Deng Xiu-Xin National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut Guo Wen-Wu National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 275-283 0167-6857 90:3<275 2007 90 11240 https://doi.org/10.1007/s11240-007-9266-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9266-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai Xiao-Dong National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Fu Jing National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Deng Xiu-Xin National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Guo Wen-Wu National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 275-283 0167-6857 90:3<275 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer