caa a22 4500 463236092 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9212-9 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9212-9 Influence of GA3, sucrose and solid medium/bioreactor culture on in vitro flowering of Spathiphyllum and association of glutathione metabolism [Elektronische Daten] [Yaser Dewir, Debasis Chakrabarty, Mohammed Ali, Nisha Singh, Eun-Joo Hahn, Kee-Yoeup Paek] Hormonal control of flower induction and inflorescence development in vitro was investigated in Spathiphyllum. The effects of gibberellic acid (GA3) and sucrose on inflorescence development were studied in plantlets regenerated in tissue culture. GA3 was mandatory for the shift from the vegetative to the reproductive stage. The effect of sucrose concentration on inflorescence bud development was studied in plantlets cultured in MS medium supplemented with 10mgl−1 GA3. Sucrose concentration at 3 or 6% induced inflorescence development in, respectively, 83-85% of the plantlets. The effect of GA3 and sucrose on inflorescence differentiation and development were also recorded in liquid culture using air-lift bioreactor. The best response was found in the same medium which was standardized as an optimum for solid culture, but the results were better than solid culture. In order to study the relationship between glutathione (GSH) and flowering, we also measured the oxidized and reduced GSH content in leaves throughout the culture period on 2 weeks interval. The GSH accumulation was more after 4 weeks until 6 weeks in GA3 treated plantlets. Similarly, glutathione reductase which is involved in the recycling of reduced GSH providing a constant intracellular level of GSH, was also higher in GA3 treated plantlets. The transient increase in GSH contents also correlated with the changes in measured γ-glutamylcysteine synthetase (γ-ECS) activity over the same period. The antioxidant enzyme activity in GA3 treated plantlets also suggests that the plants suffered increased oxidative stress during the period of GA3 treatment which subsequently increases GSH synthesis through activation of γ-ECS and this promotes flowering by increasing endogenous GSH. Springer Science+Business Media B.V., 2007 Bioreactor culture nationallicence Gibberellin nationallicence γ-Glutamylcysteine synthetase nationallicence APX : Ascorbate peroxidase nationallicence BTBB : Balloon type bubble bioreactor nationallicence CAT : Catalase nationallicence DHAR : Dehydroascorbate reductase nationallicence DTNB : 5,5′-Dithiobis-2-nitrobenzoic acid nationallicence GA : Gibberellin nationallicence GA3 : Gibberellic acid nationallicence G-POD : Guaiacol peroxidase nationallicence GR : Glutathione reductase nationallicence GSH : Glutathione nationallicence GSSG : Oxidized glutathione nationallicence MDHAR : Monodehydroascorbate reductase nationallicence MS : Murashige and Skoog's medium nationallicence PPF : Photosynthetic photon flux nationallicence SAM : Shoot apical meristem nationallicence γ-ECS : γ-Glutamylcysteine synthetase nationallicence Dewir Yaser Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut Chakrabarty Debasis Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut Ali Mohammed Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut Singh Nisha Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut Hahn Eun-Joo Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut Paek Kee-Yoeup Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 225-235 0167-6857 90:3<225 2007 90 11240 https://doi.org/10.1007/s11240-007-9212-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9212-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dewir Yaser Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut NATIONALLICENCE 700 1- Chakrabarty Debasis Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut NATIONALLICENCE 700 1- Ali Mohammed Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut NATIONALLICENCE 700 1- Singh Nisha Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut NATIONALLICENCE 700 1- Hahn Eun-Joo Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut NATIONALLICENCE 700 1- Paek Kee-Yoeup Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 225-235 0167-6857 90:3<225 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer