caa a22 4500 463236114 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9265-9 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9265-9 Efficient Agrobacterium tumefaciens -mediated transformation using embryogenic suspension cultures in sweetpotato, Ipomoea batatas (L.) Lam [Elektronische Daten] [Bo Yu, Hong Zhai, Yuping Wang, Ning Zang, Shaozhen He, Qingchang Liu] Efficient Agrobacterium tumefaciens-mediated transformation was achieved using embryogenic suspension cultures of sweetpotato (Ipomoea batatas (L.) Lam.) cv. Lizixiang. Cell aggregates from embryogenic suspension cultures were cocultivated with the A. tumefaciens strain EHA105 harboring a binary vector pCAMBIA1301 with gusA and hygromycin phosphotransferase II gene (hpt II) genes. Selection culture was conducted using 25mgl−1 hygromycin. A total of 2,218 plants were regenerated from the inoculated 1,776 cell aggregates via somatic embryogenesis. β-glucuronidase (GUS) assay and PCR, dot blot and Southern blot analyses of the regenerated plants randomly sampled showed that 90.37% of the regenerated plants were transgenic plants. The number of integrated T-DNA copies varied from 1 to 4. Transgenic plants, when transferred to soil in a greenhouse and a field, showed 100% survival. No morphological variations were observed in the ex vitro transgenic plants. These results exceed all transformation experiments reported so far in the literature in quantity of independent events per transformation experiment in sweetpotato. Springer Science+Business Media B.V., 2007 Agrobacterium tumefaciens nationallicence Embryogenic cell suspension nationallicence Genetic transformation nationallicence Sweetpotato nationallicence Transgenic plant nationallicence ABA : Abscisic acid nationallicence AS : Acetosyringone nationallicence Carb : Carbenicillin nationallicence CTAB : Cetyltrimethylammonium bromide nationallicence 2,4- d : 2,4-Dichlorophenoxyacetic acid nationallicence GUS : β-Glucuronidase nationallicence hpt II : Hygromycin phosphotransferase II gene nationallicence Hyg : Hygromycin nationallicence LB : Luria-Bertani nationallicence MS : Murashige and Skoog nationallicence npt II : Neomycin phosphotransferase II gene nationallicence Yu Bo Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut Zhai Hong Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut Wang Yuping Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut Zang Ning Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut He Shaozhen Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, 100094, Beijing, China aut Liu Qingchang Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 265-273 0167-6857 90:3<265 2007 90 11240 https://doi.org/10.1007/s11240-007-9265-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9265-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Bo Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhai Hong Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Yuping Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zang Ning Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- He Shaozhen Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Liu Qingchang Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 265-273 0167-6857 90:3<265 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer