caa a22 4500 463236122 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9254-z doi (NATIONALLICENCE)springer-10.1007/s11240-007-9254-z Agrobacterium -mediated transformation of Hyacinthus orientalis with thaumatin II gene to control fungal diseases [Elektronische Daten] [E. Popowich, A. Firsov, T. Mitiouchkina, V. Filipenya, S. Dolgov, V. Reshetnikov] Transgenic plants of hyacinth (Hyacinthus orientalis L.) cvs. Edisson and Chine Pink have been obtained by Agrobacterium-mediated transformation. Leaf explants of the both hyacinth cultivars regenerated shoots on MS medium containing 2.2μM BAP and 0.3μM NAA at a frequency of 95%. A. tumefaciens strain CBE21 carrying binary vector pBIThau35 was used for transformation. Plasmid pBIThau35 has been produced by cloning preprothaumatin II cDNA into pBI121 instead of uidA gene. Inoculated leaf explants formed calli and shoots at high frequency on selective medium with 100mgl−1 kanamycin. Four hyacinth transgenic lines of cv. Chine Pink and one line of cv. Edisson have been selected on medium containing 200mgl−1 kanamycin. The insertion of thaumatin II gene into hyacinth genome has been confirmed by PCR-analysis. All transgenic plants expressed substantial amounts of thaumatin II (between 0.06 and 0.28% of the total soluble protein). Hyacinth transgenic lines were assayed for resistance to the pathogenic fungi Fusarium culmorum and Botrytis cinerea. There were no significant differences between nontransformed control and transgenic leaves of both cultivars. At the same time the bulbs of the transgenic line Н7401 cv. Chine Pink showed the higher level of resistance to B. cinerea, the bulbs of the transgenic line Н7404 were more resistant to F. culmorum. In both cases the signs of the fungal disease were developed more slowly. The resistance of the bulbs cv. Edisson line to these fungi was not changed. All transgenic hyacinth plant were successfully transferred to soil for further evaluation. Springer Science+Business Media B.V., 2007 Botrytis cinerea nationallicence Fusarium culmorum nationallicence Hyacinth nationallicence Resistance nationallicence Transgenic plants nationallicence BAP : 6-benzylaminopurine nationallicence CaMV : Cauliflower mosaic virus nationallicence IBA : 3-indolebutyric acid nationallicence NAA : Naphthaleneacetic acid nationallicence uidA : β-glucuronidase gene nationallicence Popowich E. Central Botanic Gardens of NAS of Belarus, Surganov str. 2b, 220012, Minsk, Belarus aut Firsov A. Branch of the Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 6 Science Avenue, 142290, Pushchino, Moscow Region, Russia aut Mitiouchkina T. Branch of the Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 6 Science Avenue, 142290, Pushchino, Moscow Region, Russia aut Filipenya V. Central Botanic Gardens of NAS of Belarus, Surganov str. 2b, 220012, Minsk, Belarus aut Dolgov S. Branch of the Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 6 Science Avenue, 142290, Pushchino, Moscow Region, Russia aut Reshetnikov V. Central Botanic Gardens of NAS of Belarus, Surganov str. 2b, 220012, Minsk, Belarus aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 237-244 0167-6857 90:3<237 2007 90 11240 https://doi.org/10.1007/s11240-007-9254-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9254-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Popowich E. Central Botanic Gardens of NAS of Belarus, Surganov str. 2b, 220012, Minsk, Belarus aut NATIONALLICENCE 700 1- Firsov A. Branch of the Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 6 Science Avenue, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Mitiouchkina T. Branch of the Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 6 Science Avenue, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Filipenya V. Central Botanic Gardens of NAS of Belarus, Surganov str. 2b, 220012, Minsk, Belarus aut NATIONALLICENCE 700 1- Dolgov S. Branch of the Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 6 Science Avenue, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Reshetnikov V. Central Botanic Gardens of NAS of Belarus, Surganov str. 2b, 220012, Minsk, Belarus aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 237-244 0167-6857 90:3<237 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer