caa a22 4500 463236130 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9270-z doi (NATIONALLICENCE)springer-10.1007/s11240-007-9270-z Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae)—preliminary evidence for the symbiotic culture advantage [Elektronische Daten] [Timothy Johnson, Scott Stewart, Daniela Dutra, Michael Kane, Larry Richardson] Eulophia alta (Linnaeus) Fawcett & Rendle seeds collected from the Florida Panther National Wildlife Refuge (Collier County, FL; FPNWR) were used in a screen of five asymbiotic orchid seed germination media to determine their effectiveness in promoting germination and protocorm development. In addition, 10 fungal isolates collected from the roots of E. alta at sites in the FPNWR, Highlands County (FL), and Goethe State Forest (Levy County, FL; GSF), and a fungal isolate from the roots of Spiranthes brevilabris collected from GSF were screened for their effectiveness at promoting invitro symbiotic germination of E. alta seeds. After 18weeks asymbiotic culture, seeds sown on PhytoTechnology Orchid Seed Sowing Medium germinated to a higher percentage (87.9%) and had a higher percentage of protocorms with developing protomeristems (32.7%) than seeds cultured on Knudson C, Malmgren Modified Terrestrial Orchid Medium, ½-strength Murashige & Skoog, or Vacin & Went Modified Orchid Medium. Significantly more leaf-bearing protocorms were observed on PhytoTechnology Orchid Seed Sowing Medium (0.8%) and Vacin & Went Modified Orchid Medium (1.3%) than other media tested. Of the fungi tested, one fungal isolate (Ealt-396) promoted germination to 69.0%, two isolates promoted germination to less than 0.75% and did not support further protocorm development, and eight isolates did not support germination. Seeds co-cultured in darkness with Ealt-396 grew more rapidly than asymbiotic seedlings following germination. In addition, co-cultured (=symbiotic) seedlings continued to develop more rapidly than asymbiotic seedlings upon transfer to 16/8h light/dark photoperiod. Symbiotic seed culture of E. alta may be a more desirable method of propagation since protocorms develop more rapidly than seeds sown on asymbiotic media. Symbiotic seedlings may be more appropriate for reintroduction to natural areas than asymbiotic seedlings since symbiotic seedlings could serve to inoculate soils with a germination promoting mycobiont. Springer Science+Business Media B.V., 2007 Orchid nationallicence Seed germination nationallicence Native nationallicence Conservation nationallicence Terrestrial nationallicence Mycorrhizae nationallicence Wild coco nationallicence 1/5-PDA : 1/5-strength Potato dextrose agar nationallicence ½MS : ½-strength Murashige & Skoog nationallicence CMA : Corn meal agar nationallicence FPNWR : Florida Panther National Wildlife Refuge nationallicence dd : Distilled deionized nationallicence KC : Knudson C nationallicence L/D : Light/dark nationallicence MM : Malmgren Modified Terrestrial Orchid Medium nationallicence P723 : PhytoTechnology Orchid Seed Sowing Medium nationallicence TZ : Tetrazolium nationallicence VW : Vacin & Went Modified Orchid Medium nationallicence Johnson Timothy Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut Stewart Scott Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut Dutra Daniela Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut Kane Michael Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut Richardson Larry Florida Panther National Wildlife Refuge, U.S. Fish and Wildlife Service, 3860 Tollgate Blvd., Suite 300, 34114, Naples, FL, USA aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 313-323 0167-6857 90:3<313 2007 90 11240 https://doi.org/10.1007/s11240-007-9270-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9270-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson Timothy Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Stewart Scott Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Dutra Daniela Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Kane Michael Department of Environmental Horticulture, University of Florida, PO Box 110675, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Richardson Larry Florida Panther National Wildlife Refuge, U.S. Fish and Wildlife Service, 3860 Tollgate Blvd., Suite 300, 34114, Naples, FL, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 313-323 0167-6857 90:3<313 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer