caa a22 4500 463236149 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9269-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9269-5 Induction and comparison of different invitro morphogenesis pathways using embryo of cumin ( Cuminum cyminum L.) as a model material [Elektronische Daten] [Esmaeil Ebrahimie, Mohammad Naghavi, Abdolhadi Hosseinzadeh, Mohammad Behamta, Manijeh Mohammadi-Dehcheshmeh, Ahmad Sarrafi, German Spangenberg] In this study, using cumin embryo as explant and manipulating plant growth regulators (PGRs) in regeneration medium, the main invitro morphogenesis pathways including direct shoot organogenesis, direct somatic embryogenesis, indirect somatic embryogenesis, and indirect shoot organogenesis were obtained. The effects of PGRs, subculture, and light on the induction and progression of different pathways were studied in detail. Direct shoot organogenesis occurred on the meristematic zone, while direct somatic embryogenesis was observed on hypocotyl part of cumin embryo (more differentiated part). Application of BAP (0.1mgl−1) was the sole triggering factor for induction of callus and indirect regeneration pathways. Exogenous IAA played the central role in the direct somatic embryogenesis pathway; however, the combined effects of IAA and NAA along with the high endogenous cytokinin level resulted in direct shoot organogenesis. Subculturing revealed accelerating effects on direct somatic embryogenesis pathway and callus formation. Conversely, subculturing had negative effect on direct shoot organogenesis pathway. In certain combinations of PGRs, like 0.4mgl−1 IAA+0.4mgl−1 NAA, co-induction and co-regeneration of different pathways were observed. Investigation of genotype dependencies of different pathways showed that direct pathways are more genotype-dependent, stable, and faster than indirect pathways. This research presents the embryo of cumin as a convenient model material for induction and comparison of different morphogenesis pathways. Springer Science+Business Media B.V., 2007 Cumin nationallicence Direct and indirect regeneration nationallicence Genotype dependency nationallicence Invitro morphogenesis pathways nationallicence Organogenesis nationallicence Somatic embryogenesis nationallicence BAP : 6-benzylaminopurine nationallicence IAA : Indole-3-acetic acid nationallicence NAA : α-naphthaleneacetic acid nationallicence PGR : Plant growth regulator nationallicence Ebrahimie Esmaeil Department of Crop Production and Plant Breeding, Faculty of Agriculture, University of Shiraz, Shiraz, Iran aut Naghavi Mohammad Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran aut Hosseinzadeh Abdolhadi Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran aut Behamta Mohammad Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran aut Mohammadi-Dehcheshmeh Manijeh Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran aut Sarrafi Ahmad Laboratoire de Biotechnologie, et Amelioration des Plantes, Castanet cedex, France aut Spangenberg German Plant Biotechnology Centre, Victorian AgriBiosciences Centre, LaTrobe R&D Park, Bundoora, VIC, Australia aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 293-311 0167-6857 90:3<293 2007 90 11240 https://doi.org/10.1007/s11240-007-9269-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9269-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ebrahimie Esmaeil Department of Crop Production and Plant Breeding, Faculty of Agriculture, University of Shiraz, Shiraz, Iran aut NATIONALLICENCE 700 1- Naghavi Mohammad Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Hosseinzadeh Abdolhadi Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Behamta Mohammad Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Mohammadi-Dehcheshmeh Manijeh Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Sarrafi Ahmad Laboratoire de Biotechnologie, et Amelioration des Plantes, Castanet cedex, France aut NATIONALLICENCE 700 1- Spangenberg German Plant Biotechnology Centre, Victorian AgriBiosciences Centre, LaTrobe R&D Park, Bundoora, VIC, Australia aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 293-311 0167-6857 90:3<293 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer