caa a22 4500 463236157 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9263-y doi (NATIONALLICENCE)springer-10.1007/s11240-007-9263-y Regeneration of doubled haploid plants by androgenesis of cucumber ( Cucumis sativus L.) [Elektronische Daten] [Hui Song, Qun-Feng Lou, Xiang-Dong Luo, Joseph Wolukau, Wei-Ping Diao, Chun-Tao Qian, Jin-Feng Chen] Six experiments (including pretreatment, embryonic callus induction media, preculture conditions, embryo induction media, embryo germination media, and genotypic effects) were conducted to develop an efficient cucumber (Cucumis sativus L., 2n=2x=14) anther culture protocol. Pretreatment and embryo induction were key factors for successful anther culture. Suitable temperature stress depended on the ecotype, i.e., cucumbers from cold areas responded well to cold shock whereas those from temperate areas responded well to heat treatment. The best medium for embryonic callus induction was MS medium supplemented with 4.44μM BA, 2.26μM 2, 4-D, 4.64μM KIN, 3% sucrose and 0.8% agar. For embryo induction, MS medium supplemented with 0.54μM NAA, 13.32μM BA, 3% sucrose and 0.8% agar was optimal, and for embryo germination MS medium containing 2.22μM BA, 6% sucrose and 1.2% agar was best. Using this protocol, we produced callus from 16 genotypes and regenerated plants from three of 20 evaluated. Three embryos per anther and 42 DH per 45 anthers (93% success) were obtained for cv. Ningjia No. 1, which was an improved result over a previous report. The origin of regenerants from microspores was determined by cytological, morphological and AFLP analyses. Springer Science+Business Media B.V., 2007 Anther culture nationallicence Inbred lines nationallicence Cucurbitaceae nationallicence R0 : Anther culture derived plants nationallicence R1 : Selfed progeny of R0 nationallicence DH : Doubled haploid nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence BA : 6-benzyladenine nationallicence KIN : Kinetin nationallicence TDZ : Thidiazuron nationallicence NAA : α-naphthaleneacetic acid nationallicence MS : Murashige and Skoog (1962) nationallicence PGR : Plant growth regulator nationallicence Song Hui State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Lou Qun-Feng State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Luo Xiang-Dong State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Wolukau Joseph State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Diao Wei-Ping State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Qian Chun-Tao State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Chen Jin-Feng State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 245-254 0167-6857 90:3<245 2007 90 11240 https://doi.org/10.1007/s11240-007-9263-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9263-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Hui State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Lou Qun-Feng State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Luo Xiang-Dong State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Wolukau Joseph State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Diao Wei-Ping State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Qian Chun-Tao State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Chen Jin-Feng State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 245-254 0167-6857 90:3<245 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer