caa a22 4500 463236165 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11240-007-9264-x doi (NATIONALLICENCE)springer-10.1007/s11240-007-9264-x Variable DNA content of Cyclamen persicum regenerated via somatic embryogenesis: rethinking the concept of long-term callus and suspension cultures [Elektronische Daten] [Thomas Borchert, Jörg Fuchs, Traud Winkelmann, Annette Hohe] By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation for the absolute DNA content in the range of 3.17pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes. Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework of future application of invitro propagation techniques based on regeneration from fastly growing undifferentiated cells like long-term callus and suspension cultures. Springer Science+Business Media B.V., 2007 Flow cytometry nationallicence DNA amount nationallicence DNA-Index nationallicence Micropropagation nationallicence Somaclonal variation nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence 2-iP : 6-(γ,γ-Dimethylallyl-amino)purine nationallicence CV : Coefficient of variation nationallicence DAPI : 4′,6-Diamidino-2-phenylindol nationallicence DI : DNA-index nationallicence PI : Propidium iodide nationallicence Borchert Thomas Department of Hospital and Clinical Engineering, Environment and Biotechnology, University of Applied Sciences Giessen, Wiesenstrasse 14, 35390, Giessen, Germany aut Fuchs Jörg Leibniz-Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut Winkelmann Traud Institute of Floriculture and Woody Plant Science, Leibniz Universität Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany aut Hohe Annette Department of Plant Propagation, Institute of Vegetable and Ornamental Crops, Kuehnhaeuser Strasse 101, 99189, Erfurt-Kuehnhausen, Germany aut Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 255-263 0167-6857 90:3<255 2007 90 11240 https://doi.org/10.1007/s11240-007-9264-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9264-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borchert Thomas Department of Hospital and Clinical Engineering, Environment and Biotechnology, University of Applied Sciences Giessen, Wiesenstrasse 14, 35390, Giessen, Germany aut NATIONALLICENCE 700 1- Fuchs Jörg Leibniz-Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Winkelmann Traud Institute of Floriculture and Woody Plant Science, Leibniz Universität Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany aut NATIONALLICENCE 700 1- Hohe Annette Department of Plant Propagation, Institute of Vegetable and Ornamental Crops, Kuehnhaeuser Strasse 101, 99189, Erfurt-Kuehnhausen, Germany aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 90/3(2007-09-01), 255-263 0167-6857 90:3<255 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer