caa a22 4500 463236173 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-006-9163-6 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9163-6 Screening and selecting arbuscular mycorrhizal fungi for inoculating micropropagated apple rootstocks in acid soils [Elektronische Daten] [José Cavallazzi, Osmar Filho, Sidney Stürmer, Paul Rygiewicz, Margarida de Mendonça] Santa Catarina state is the largest producer of apples in Brazil. Soils in this region have low pH and high levels of aluminum and manganese, requiring high inputs of fertilizers and amendments increasing costs of apple production. Inoculation of arbuscular mycorrhizal fungi can improve the establishment of micropropagated apple plants in such adverse soil conditions. Soil samples were collected from apple orchards in the Caçador, Fraiburgo and São Joaquim regions to develop a corn bioassay to identify mycorrhizal communities with high infectivity. Eleven fungal species were identified from one Caçador soil with the highest infectivity. Glomus etunicatum SCT110, Scutellospora pellucida SCT111, Acaulospora scrobiculata SCT112 and Scutellospora heterogama SCT113 were brought into single-species culture and used in a plant growth and nutrient uptake experiment using micropropagated apple (Malus prunifolia), cultivated at three soil pH. Colonization by fungal isolates significantly affected plant height, shoot and root dry weights, and root:shoot ratio. Soil pH also significantly affected all growth parameters except shoot dry weight. Mycorrhizal inoculation also significantly altered tissue concentrations of P, Zn, Cu, Ca, S, Na, N, K, Fe and Al. Association with mycorrhizal fungi increased P concentration and also decreased Al concentrations in the shoots. Overall, G. etunicatum and S. pellucida were the most effective isolates to promote plant growth and nutrient uptake. Inoculation of apple rootstock with selected fungal isolates during the acclimatization stage represents a useful strategy for producing micropropagated apples that can withstand acidic soil conditions. Springer Science+Business Media B.V., 2007 Acid soils nationallicence Aluminum nationallicence Brazil nationallicence Micropropagated apple nationallicence Mycorrhizae nationallicence Plant-microorganisms interaction nationallicence Nutrient content nationallicence Plant growth nationallicence Cavallazzi José Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Cx. P. 476, 88040-900, Florianopolis, SC, Brazil aut Filho Osmar Departamento de Solos, Universidade do Estado de Santa Catarina, Cx. P. 281, 88520-000, Lages, SC, Brazil aut Stürmer Sidney Departamento de Ciências Naturais, Universidade Regional de Blumenau, Cx. P. 1507, 89010-971, Blumenau, SC, Brazil aut Rygiewicz Paul Western Ecology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 97333, Corvallis, OR, USA aut de Mendonça Margarida Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Cx. P. 476, 88040-900, Florianopolis, SC, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 117-129 0167-6857 90:2<117 2007 90 11240 https://doi.org/10.1007/s11240-006-9163-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9163-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cavallazzi José Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Cx. P. 476, 88040-900, Florianopolis, SC, Brazil aut NATIONALLICENCE 700 1- Filho Osmar Departamento de Solos, Universidade do Estado de Santa Catarina, Cx. P. 281, 88520-000, Lages, SC, Brazil aut NATIONALLICENCE 700 1- Stürmer Sidney Departamento de Ciências Naturais, Universidade Regional de Blumenau, Cx. P. 1507, 89010-971, Blumenau, SC, Brazil aut NATIONALLICENCE 700 1- Rygiewicz Paul Western Ecology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 97333, Corvallis, OR, USA aut NATIONALLICENCE 700 1- de Mendonça Margarida Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Cx. P. 476, 88040-900, Florianopolis, SC, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 117-129 0167-6857 90:2<117 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer