caa a22 4500 463236181 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-006-9191-2 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9191-2 Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania Somnifera (L.) Dunal. plantlets [Elektronische Daten] [Sang-Ho Lee, Rajesh Tewari, Eun-Joo Hahn, Kee-Yoeup Paek] The aim of the study was to establish whether the quantity and the quality of light affect growth and development of Withania somnifera plantlets. We have studied growth and histo-physiological parameters [stomatal characteristics, chloroplastic pigments concentrations, photosynthesis, and transpiration (E)] of W. somnifera plantlets regenerated under various light intensities, or monochromatic light or under a mixture of two colors of light in tissue culture conditions. Plantlets grown under a photon flux density (PFD) of 30μmolm-2s-1 showed greater growth and development than those raised under other PFDs. Chlorophylls and carotenoids, numbers of stomata, rate of photosynthesis (PN) and transpiration (E), stomatal conductance (gs), and water use efficiency (WUE) increased with increasing the PFD up to 60μmolm-2s-1. Light quality also affected plantlets growth and physiology. Highest growth was observed under fluorescent and in a mixture of blue and red light. Very few stomata were developed in any of the monochromatic light but under fluorescent or under a mixture of two colors stomatal numbers increased. Similarly, gs, E, PN, and WUE were also higher under fluorescent light and under a mixture of red and blue light. Regressional analysis showed a linear relationship between PN (r 2=70) and gs and between E (r 2=0.95) and gs. In conclusion, both the quality and the quantity of light affect growth of plantlets, development of stomata and physiological responses differently depending on the intensity and the wavelength of light. Springer Science+Business Media B.V., 2007 Ashwagandha nationallicence Indian ginseng nationallicence In-vitro culture nationallicence Photosynthesis nationallicence Spectral intensity nationallicence Spectral quality nationallicence Stomatal development nationallicence Transpiration nationallicence Water use efficiency nationallicence E : Transpiration nationallicence EDTA : Ethylenediamine tetraacetic acid nationallicence gs : Stomatal conductance nationallicence LEDs : Light emitting diodes nationallicence LHC : Light harvesting complex nationallicence PFD : Photon flux density nationallicence PN : Rate of photosynthesis nationallicence POD : Peroxidase nationallicence WUE : Water use efficiency nationallicence Lee Sang-Ho Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut Tewari Rajesh Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut Hahn Eun-Joo Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut Paek Kee-Yoeup Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 141-151 0167-6857 90:2<141 2007 90 11240 https://doi.org/10.1007/s11240-006-9191-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9191-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Sang-Ho Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut NATIONALLICENCE 700 1- Tewari Rajesh Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut NATIONALLICENCE 700 1- Hahn Eun-Joo Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut NATIONALLICENCE 700 1- Paek Kee-Yoeup Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 141-151 0167-6857 90:2<141 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer