caa a22 4500 463236203 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-007-9235-2 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9235-2 Invitro morphogenesis patterns from shoot apices of sugar cane are determined by light and type of growth regulator [Elektronische Daten] [Renata Garcia, Daniela Cidade, Aline Castellar, Andrea Lips, Claudia Magioli, Catia Callado, Elisabeth Mansur] The regeneration patterns of shoot apices derived from invitro plants of four varieties of sugar cane in response to different growth regulators and light were evaluated. The cellular origin of the regeneration processes was also investigated. Explants cultivated on medium supplemented with NAA and incubated under light showed direct bud regeneration from cells of external layers of the ground parenchyma of the stem. Explants cultivated in the dark on medium supplemented with low concentrations of picloram (PIC) or 2,4D (4.0 and 4.5μM, respectively) showed callus formation derived from the ground parenchyma of stem and development of preembryogenic masses derived from bundle sheath cells facing the phloem tissue of immature leaves. Somatic embryos at further developmental stages were visible following transfer to medium devoid of growth regulators and incubation under light. When incubated under light since the begining of the experiment, explants cultivated in the presence of higher PIC or 2,4D concentrations (40 and 22.6μM, respectively) first displayed direct organogenesis from external layers of the ground parenchyma of the stem, followed by the development of organogenic calluses. Preembryogenic masses were also observed from bundle sheath cells of immature leaves. However, in contrast to the cultures pre-incubated in darkness for 30days, the subsequent stages of embryo development were not detected. The regeneration efficiency of calluses induced by 2,4D and PIC was generally increased following desiccation in laminar flow or incubation on medium solidified with phytagel. Springer Science+Business Media B.V., 2007 Sugarcane nationallicence Shoot apices nationallicence Micropropagation nationallicence Embryogenesis nationallicence Organogenesis nationallicence Garcia Renata Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Cidade Daniela Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Castellar Aline Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Lips Andrea Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Magioli Claudia Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Callado Catia Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Mansur Elisabeth Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 181-190 0167-6857 90:2<181 2007 90 11240 https://doi.org/10.1007/s11240-007-9235-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9235-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Garcia Renata Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Cidade Daniela Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Castellar Aline Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Lips Andrea Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Magioli Claudia Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Callado Catia Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Mansur Elisabeth Universidade do Estado do Rio de Janeiro, 20550-013, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 181-190 0167-6857 90:2<181 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer