caa a22 4500 463236211 CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-007-9252-1 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9252-1 Shoot regeneration from organogenic callus of sweet cherry ( Prunus avium L.) [Elektronische Daten] [Mistianne Feeney, Basdeo Bhagwat, Jeremy Mitchell, W. Lane] A complete method to regenerate adventitious shoots and to produce field-ready trees from three commercial cultivars of sweet cherry (Prunus avium L.) is described. The effects of explant types, pre-treatments, basal media, and phloroglucinol on cultivars Bing, Sweetheart, and Lapins were investigated. Callus developed on four explant types: apical shoot tips isolated from orchard trees; and punctured shoot tips, stem sections, and shoot bases of in vitro shoot cultures. Callus formed on Bing (5%), Sweetheart (8%), and Lapins (20%) shoot tips from orchard trees after 4months on Murashige and Skoog medium (MS) at half-strength with 3μM benzylaminopurine (BA). In vitro-derived explants formed callus after 3months on Woody Plant Medium with 3μM BA (W3B): punctured shoot tips (Sweetheart and Lapins 67%), stem sections (Sweetheart 31%, Lapins 27%), and shoot bases (Sweetheart 10%, Lapins 17%). Pre-treatment of shoot cultures on MS with 3μM BA and 1mM phloroglucinol increased callus formation three-fold on shoot base explants. Callus was separated from parental explants and maintained on MS with 3μM BA. Shooting was induced by transferring callus to W3B. At 2weeks, shoot development approached 100%. By 4weeks, 7-17 shoots had formed on each explant. Callus was maintained for 1.5years with no decrease in shoot production. Shoots were grafted onto Mazzard (P. avium) rootstocks with 54% (Sweetheart), 57% (Lapins), and 21% (Bing) success after 5weeks. Springer Science+Business Media B.V., 2007 Adventitious shoots nationallicence Basal medium nationallicence Fruit tree nationallicence Grafting nationallicence Phloroglucinol nationallicence BA : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence IBA : Indole-3-butyric acid nationallicence MS : Murashige and Skoog medium nationallicence NAA : α-naphthaleneacetic acid nationallicence WPM : Woody Plant Medium nationallicence Feeney Mistianne Okanagan Specialty Fruits Inc., PO Box 1533, V0H 1Z0, Summerland, British Columbia, Canada aut Bhagwat Basdeo Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, V0H 1Z0, Summerland, British Columbia, Canada aut Mitchell Jeremy Department of Behavioural Ecology, Institute of Zoology, University of Bern, 3032, Bern, Switzerland aut Lane W. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, V0H 1Z0, Summerland, British Columbia, Canada aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 201-214 0167-6857 90:2<201 2007 90 11240 https://doi.org/10.1007/s11240-007-9252-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9252-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feeney Mistianne Okanagan Specialty Fruits Inc., PO Box 1533, V0H 1Z0, Summerland, British Columbia, Canada aut NATIONALLICENCE 700 1- Bhagwat Basdeo Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, V0H 1Z0, Summerland, British Columbia, Canada aut NATIONALLICENCE 700 1- Mitchell Jeremy Department of Behavioural Ecology, Institute of Zoology, University of Bern, 3032, Bern, Switzerland aut NATIONALLICENCE 700 1- Lane W. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, V0H 1Z0, Summerland, British Columbia, Canada aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 201-214 0167-6857 90:2<201 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer