caa a22 4500 46323622X CHVBK 20180405153257.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-006-9181-4 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9181-4 High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl [Elektronische Daten] [Peng Zhao, Wei Wang, Fo-Sheng Feng, Fei Wu, Zhong-Qi Yang, Wan-Jun Wang] An efficient in vitro propagation protocol for Dendrobium candidum Wall ex Lindl. using transverse thin cell layer (tTCL) culture system was established. The frequency of shoot regeneration and the number of adventitious buds produced from the regenerated shoots significantly relied on the concentration of plant growth regulators, and the position and orientation of the explant. Murashige and Skoog (MS) medium with half-strength macronutrients and 2% sucrose, supplemented with 1.2mgl−1 naphthaleneacetic acid (NAA) and 1.2mgl−1 6-benzyladenine (6-BA), was optimal for shoot regeneration. Upon this medium, the youngest explant inoculated in the upright orientation exhibited a high frequency of shoot regeneration (92%), and the highest number of adventitious buds (an average of 24.5) per explant. Rooting of shoots and adventitious buds was achieved on MS medium with half-strength macronutrients and 2% sucrose with 1.0mgl−1 NAA and 1.0mgl−1 indole-3-acetic acid (IAA). Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Ontogenetic studies revealed that the shoots originated from the stem vascular bundles. Springer Science+Business Media B.V., 2007 Dedifferentiation nationallicence Morphogenesis nationallicence Orientation nationallicence Position nationallicence Transverse thin cell layer (tTCL) nationallicence Vascular bundle nationallicence 6-BA : 6-benzyladenine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence IAA : Indole-3-acetic acid nationallicence Kin : Kinetin nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : Naphthaleneacetic acid nationallicence PLBs : Protocorm-like bodies nationallicence tTCL : Transverse thin cell layer nationallicence Zhao Peng College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut Wang Wei College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut Feng Fo-Sheng College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut Wu Fei College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut Yang Zhong-Qi College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut Wang Wan-Jun College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 131-139 0167-6857 90:2<131 2007 90 11240 https://doi.org/10.1007/s11240-006-9181-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9181-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Peng College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut NATIONALLICENCE 700 1- Wang Wei College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut NATIONALLICENCE 700 1- Feng Fo-Sheng College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut NATIONALLICENCE 700 1- Wu Fei College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut NATIONALLICENCE 700 1- Yang Zhong-Qi College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut NATIONALLICENCE 700 1- Wang Wan-Jun College of Bio-engineering, Southwest Jiaotong University, 610031, Chengdu, Sichuan province, The People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 131-139 0167-6857 90:2<131 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer