caa a22 4500 463236238 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-007-9261-0 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9261-0 Rose-scented geranium ( Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality [Elektronische Daten] [Gauri Saxena, Suchitra Banerjee, Laiq-ur-Rahman,, Praveen Verma, G. Mallavarapu, Sushil Kumar] Transgenic plants of rose-scented geranium (Pelargonium graveolens cv. Hemanti) have been produced from Agrobacterium rhizogenes (strains A4 and LBA9402) mediated hairy root cultures. Amongst the explants tested, leaves were most responsive followed by the petioles and internodal segments, respectively. The A4 strain performed better for all the three explants both in terms of frequency of response and time requirement for hairy root induction. Transgenic shoots could be obtained by spontaneous regeneration without intervening callus phase amongst 16% and 12% root lines of A4 and LBA 9402 origin, respectively, or they were induced in 29% and 22% hairy root lines of A4 and LBA9402 origin, respectively, with different hormonal supplementation. These transgenic plants showed 30% survival as against 90% of their control under the confined environment of glasshouse. The transgenic plants were of similar morphotype having increased branching, higher number of leaves with increased dentations, short and round stature, highly branched root system and absence of leaf wrinkling. These transgenic plants showed opine positive results even after 5months of their transfer to the glasshouse. The essential oil compositions of 81% of these transgenics were qualitatively similar to that of the wild type parent. However, two transgenic plants (LZ-3 and 14TG) showed increase in concentrations of geraniol and geranyl esters signifying improved oil quality with respect to the citronellol:geraniol ratio. These two oils having better olfactory value represent an improvement over that of the wild type parent from the commercial point of view. Springer Science+Business Media B.V., 2007 Geranium nationallicence Pelargonium nationallicence Agrobacterium rhizogenes nationallicence Essential oil nationallicence MS : Murashige and Skoog's medium nationallicence IBA : Indole butyric acid nationallicence BAP : 6-Benzylaminopurine nationallicence NAA : Naphthaleneacetic acid nationallicence GC : Gas chromatography nationallicence GC/MS : Gas chromatography mass spectroscopy nationallicence Saxena Gauri Department of Botany, Lucknow University, 226001, Lucknow, India aut Banerjee Suchitra Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India aut Laiq-ur-Rahman, CIMAP Field Station, Puraura, India aut Verma Praveen National Botanical Research Institute, Rana Pratap Marg, 226001, Lucknow, India aut Mallavarapu G. CIMAP Field Station, Bangalore, India aut Kumar Sushil National Centre for Plant Genome Research, JNU Campus, P.O. Box 10531, 110067, New Delhi, India aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 215-223 0167-6857 90:2<215 2007 90 11240 https://doi.org/10.1007/s11240-007-9261-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9261-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saxena Gauri Department of Botany, Lucknow University, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Banerjee Suchitra Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India aut NATIONALLICENCE 700 1- Laiq-ur-Rahman CIMAP Field Station, Puraura, India aut NATIONALLICENCE 700 1- Verma Praveen National Botanical Research Institute, Rana Pratap Marg, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Mallavarapu G. CIMAP Field Station, Bangalore, India aut NATIONALLICENCE 700 1- Kumar Sushil National Centre for Plant Genome Research, JNU Campus, P.O. Box 10531, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 215-223 0167-6857 90:2<215 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer