caa a22 4500 463236246 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s11240-007-9241-4 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9241-4 Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis [Elektronische Daten] [Wang Xiao, Xue-Lin Huang, Xia Huang, Ya-Ping Chen, Xue-Mei Dai, Jie-Tang Zhao] A protocol for plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis was developed. Viable protoplasts were isolated from embryogenic cell suspensions at a yield of 1.2×107 protoplasts/ml packed cell volume (PCV). Liquid and feeder layer culture systems with medium-A and medium-B were used for protoplast culture. In liquid culture system, medium-B was more efficient for inducing cell division (17.5% at 14days) and colony formation (6.7% at 28days) than medium-A. However, all protoplast-derived cell colonies (PDCC) obtained from liquid culture system could not develop further. In feeder layer culture system, there was no significant difference between medium-A and medium-B on cell division and colony formation of the cultured protoplasts, and the cell division frequency at 14days and colony formation frequency at 28days were 24.5% and 11.2%, respectively, in medium-B. Comparative study on the effects of BAP (2.2μM, 4.4μM, 8.8μM), zeatin (0.4μM, 0.8μM, 1.2μM) and TDZ (0.2μM, 0.4μM, 0.6μM) on embryo formation of PDCC from feeder-layer culture indicated that TDZ was best. TDZ at 0.4μM induced 7906 mature embryos per ml PCV PDCC, which was 4-fold the frequency as with BAP at 4.4μM, 7.5-fold as with zeatin at 0.8μM and 150-fold as control medium (no mentioned cytokinins) after 45days on M3 medium. About 44% of the mature embryos were converted into plantlets with poor root system after subculture on M4 medium. Root further development of regenerated plantlets was promoted by addition of activated charcoal (AC) to MS basal medium. Springer Science+Business Media B.V., 2007 Musa acuminata cv. Mas (AA) nationallicence Protoplast culture nationallicence Somatic embryogenesis nationallicence Cytokinin nationallicence Plant regeneration nationallicence AC : Activated charcoal nationallicence BAP : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence ECS : Embryogenic cell suspensions nationallicence FDA : Fluorescein diacetate nationallicence Gln : Glutamine nationallicence IAA : Indolacetic-3-acid nationallicence MES : 2-N-morpholino ethanesulfonic acid nationallicence NAA : 1-naphthaleneacetic acid nationallicence PCV : Packed cell volume nationallicence PDCC : Protoplast-derived cell colonies nationallicence TDZ : Thidiazuron nationallicence Xiao Wang The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut Huang Xue-Lin The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut Huang Xia The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut Chen Ya-Ping The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut Dai Xue-Mei The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut Zhao Jie-Tang The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 191-200 0167-6857 90:2<191 2007 90 11240 https://doi.org/10.1007/s11240-007-9241-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9241-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao Wang The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Huang Xue-Lin The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Huang Xia The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Chen Ya-Ping The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Dai Xue-Mei The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Zhao Jie-Tang The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, Guangdong, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 90/2(2007-08-01), 191-200 0167-6857 90:2<191 2007 90 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer