caa a22 4500 463236262 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9209-4 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9209-4 Application of embryo rescue after interspecific crosses in the genus Rhododendron [Elektronische Daten] [Tom Eeckhaut, Ellen De Keyser, Johan Van Huylenbroeck, Jan De Riek, Erik Van Bockstaele] Interspecific hybridization between evergreen pot azalea (Tsutsusi) cultivars and genotypes of other Rhododendron subgenera or sections (Rhododendron, Hymenanthes, Pentanthera, Vireya) is significantly hampered by many prezygotic and postzygotic barriers. The objective of our work was to overcome spontaneous abortion and lack of endosperm formation and to increase germination rates by establishing an embryo rescue protocol. The optimal germination medium for immature Rhododendron seeds was a basal medium supplemented with 145μM GA3. This medium induced germination of fertilized ovules after several sexual combinations of subgenera. Its use was clearly more efficient than in vivo sowing. The direction of the cross significantly influenced the occurrence of abortion, germination and albinism. The obtained seedlings were multiplied on Woody Plant Medium+4.5μM 2iP, and rooted afterwards. Finally, about 9% of the germinated ovules resulted in vigorously growing seedlings that were successfully acclimatized. Springer Science+Business Media B.V., 2007 Azalea nationallicence Germination nationallicence Gibberellins nationallicence Incongruity nationallicence Invitro nationallicence Ovule culture nationallicence Tsutsusi nationallicence 2iP : Isopentenyladenine nationallicence GA : Gibberellic acid nationallicence HY : Subgenus Hymenanthes nationallicence IAA : Indole acetic acid nationallicence MS : Murashige & Skoog nationallicence PE : Subgenus Pentanthera nationallicence RH : Subgenus Rhododendron nationallicence TS : Subgenus Tsutsusi nationallicence VI : Section Vireya nationallicence Eeckhaut Tom Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut De Keyser Ellen Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Van Huylenbroeck Johan Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut De Riek Jan Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Van Bockstaele Erik Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 29-35 0167-6857 89:1<29 2007 89 11240 https://doi.org/10.1007/s11240-007-9209-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9209-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eeckhaut Tom Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- De Keyser Ellen Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Van Huylenbroeck Johan Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- De Riek Jan Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 700 1- Van Bockstaele Erik Unit Plant-Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 29-35 0167-6857 89:1<29 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer