caa a22 4500 463236270 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9210-y doi (NATIONALLICENCE)springer-10.1007/s11240-007-9210-y Somatic embryogenesis of Myrciaria aureana (Brazilian grape tree) [Elektronische Daten] [Sergio Motoike, Edson Saraiva, Marilia Ventrella, Crislene Silva, Luiz Salomão] The aim of this research was to establish a long-term somatic embryogenic cultures that could be used for cryopreservation. For the induction of somatic embryogenesis, different levels of 2,4-D as well as the combination of 2,4-D and indole-3-acetyl-l-aspartic acid (IASP) were tested on cotyledons of zygotic embryos. The somatic embryogenic cultures were established and maintained up to 2years through frequent subculturing on a medium containing 2,4D+IASP. Light, activated charcoal, and polyethylene glycol (PEG) were tested for the regeneration and maturation of somatic embryos, and the mature embryos were germinated in JADS medium. The combination of light and PEG provided the highest number of mature embryos. The somatic embryos obtained were smaller than zygotic embryos and lacked starch. There was an interaction between 2,4-D and IASP on the induction and regeneration of somatic embryo in Myrciaria aureana. The combination of light and PEG increased the number of mature embryos; however, charcoal was detrimental to the process. Springer Science+Business Media B.V., 2007 Charcoal nationallicence In vitro culture nationallicence Jaboticaba nationallicence Light nationallicence Myrtaceae nationallicence Polyethylene glycol nationallicence Ch : Activated charcoal nationallicence BM : Basal medium nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence IASP : Indole-3-acetyl-l-aspartic acid nationallicence IBA : Indole-3-butyric acid nationallicence JADS : Correia etal. (1995) medium nationallicence NN : Nitsch and Nitsch (1969) medium nationallicence PAR : Photosynthetically active radiation nationallicence PEG : Polyethylene glycol nationallicence PEM : Pro-embryogenic mass nationallicence PVP : Polyvinyl-pyrrolidone nationallicence RM1 : Regeneration medium 1 nationallicence RM2 : Regeneration medium 2 nationallicence Motoike Sergio Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut Saraiva Edson Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut Ventrella Marilia Departamento de Biologia Vegetal, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut Silva Crislene Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut Salomão Luiz Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 75-81 0167-6857 89:1<75 2007 89 11240 https://doi.org/10.1007/s11240-007-9210-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9210-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Motoike Sergio Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Saraiva Edson Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Ventrella Marilia Departamento de Biologia Vegetal, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Silva Crislene Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Salomão Luiz Departamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 75-81 0167-6857 89:1<75 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer