caa a22 4500 463236289 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9207-6 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9207-6 Somatic embryogenesis from immature peach palm inflorescence explants: towards development of an efficient protocol [Elektronische Daten] [Douglas Steinmacher, Charles Clement, Miguel Guerra] Various factors affect the induction of somatic embryogenesis in peach palm (Bactris gasipaes Kunth). Among these, both the type and level of auxins had the greatest influence on in vitro responses, although the genotype and the developmental stage of the explants also influenced results. Younger inflorescences were more competent to respond to SE induction than more mature inflorescences and the use of a pre-treatment with 2,4-D (200μM) in liquid MS culture medium also increased the embryogenic capacity, and diminished the development of flower buds. Higher oxidation rates were observed in explants maintained on 2,4-D-supplemented culture medium, while on 300μM or 600μM Picloram and Dicamba lower oxidation rates were observed. The progression from floral meristem to flower bud occurred at high frequency when low concentrations of auxins were used, independent of the type. Higher concentrations of Picloram or Dicamba reduced or even inhibited flower bud development. Picloram also enhanced the embryogenic induction rate more than 2,4-D and Dicamba, and among the concentrations evaluated 300μM Picloram enhanced induction for both genotypes, with significant differences between genotypes. The best combination of variables used the least mature inflorescence (Infl1) from genotype I with the 2,4-D pre-treatment and 300μM Picloram to generate 5 embryogenic calli from 18 explants; 26 embryos were obtained on average from each embryogenic callus. From these, eighteen embryos converted to plantlets and six of these survived transfer to the greenhouse. Springer Science+Business Media B.V., 2007 Arecaceae nationallicence Bactris gasipaes Kunth nationallicence Tissue culture nationallicence Clonal propagation nationallicence Auxin nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence 2-iP : 2-isopentyladenine (6-dimethylaminopurine) nationallicence Dicamba : 3,6-dichloro-2-methoxybenzoic acid nationallicence MS : Murashige and Skoog's salts nationallicence NAA : Naphthalene acetic acid nationallicence Picloram : 4-amino-3,5,6-trichloropicolinic acid nationallicence SE : Somatic embryogenesis nationallicence Steinmacher Douglas Dept Fitotecnia/LFDGV, UFSC, Universidade Federal de Santa Catarina, 88040-900, Florianopolis, SC, Brazil aut Clement Charles INPA, Instituto Nacional de Pesquisas da Amazônia, Cx Postal 478, 69011-970, Manaus, Amazonas, Brazil aut Guerra Miguel Dept Fitotecnia/LFDGV, UFSC, Universidade Federal de Santa Catarina, 88040-900, Florianopolis, SC, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 15-22 0167-6857 89:1<15 2007 89 11240 https://doi.org/10.1007/s11240-007-9207-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9207-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steinmacher Douglas Dept Fitotecnia/LFDGV, UFSC, Universidade Federal de Santa Catarina, 88040-900, Florianopolis, SC, Brazil aut NATIONALLICENCE 700 1- Clement Charles INPA, Instituto Nacional de Pesquisas da Amazônia, Cx Postal 478, 69011-970, Manaus, Amazonas, Brazil aut NATIONALLICENCE 700 1- Guerra Miguel Dept Fitotecnia/LFDGV, UFSC, Universidade Federal de Santa Catarina, 88040-900, Florianopolis, SC, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 15-22 0167-6857 89:1<15 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer