caa a22 4500 463236297 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9208-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9208-5 Micrografting of Protea cynaroides [Elektronische Daten] [H. Wu, E. du Toit, C. Reinhardt] The inability to induce rooting of in vitro-established Protea cynaroides microshoots has prevented the production of complete plantlets. A successful shoot-tip micrografting technique was developed using in vitro-germinated P. cynaroides seedlings as rootstocks and axenic microshoots established from pot plants as microscions. Thirty-day old seedlings, germinated on growth-regulator-free, half-strength Murashige and Skoog medium, were decapitated and a vertical incision made from the top end. The bottom ends of microshoots established on modified Murashige and Skoog medium were cut into a wedge (‘V') shape, and placed into the incision. The micrografted explants were cultured in a growth chamber with the temperature adjusted to 25±2°C, with a 12-h photoperiod. Best results were obtained by placing the microscions directly onto the rootstock without any pre-treatments. Dipping the explants in anti-oxidant solution or placing a layer of medium around the graft area led to the blackening of the microscion. Springer Science+Business Media B.V., 2007 Grafting nationallicence In vitro nationallicence King Protea nationallicence Micropropagation nationallicence Proteaceae nationallicence EDTA : Ethylenediaminetetraacetate nationallicence BAP : 6-Benzylaminopurine nationallicence GA3 : Gibberellic acid nationallicence PAR : Photosynthetic active radiation nationallicence Wu H. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa aut du Toit E. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa aut Reinhardt C. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 23-28 0167-6857 89:1<23 2007 89 11240 https://doi.org/10.1007/s11240-007-9208-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9208-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu H. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa aut NATIONALLICENCE 700 1- du Toit E. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa aut NATIONALLICENCE 700 1- Reinhardt C. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 23-28 0167-6857 89:1<23 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer