caa a22 4500 463236300 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9211-x doi (NATIONALLICENCE)springer-10.1007/s11240-007-9211-x Characterization of Anthocyanidin Synthase (ANS) Gene and anthocyanidin in rare medicinal plant- Saussurea medusa [Elektronische Daten] [Liqin Cheng, Yanjun Xu, Erich Grotewold, Zhiping Jin, Fengyan Wu, Chunxiang Fu, Dexiu Zhao] Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzes the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. Here, we report the cloning and characterization of an anthocyanidin Synthase gene (SmANS1) from a cDNA library derived from a red callus line of Saussurea medusa, a traditional Chinese medicinal plant. SmANS1 exhibits significant similarities in amino acid sequence with members of a family of 2-oxoglutarate-dependent oxygenases. The deduced SmANS1 protein shared 83% and 79% identity with that of Callistephus chinensis and Gerbera hybrids, respectively. Reverse transcription-PCR (RT-PCR) analyses of SmANS1 transcripts showed that the SmANS1 was abundantly expressed in the cell cultures of red cell line and seedlings, and weakly expressed in the counterparts of yellow cell line, but was not expressed in the roots. Further more, the anthocyanins composition of cell cultures from Saussurea medusa was investigated using high-performance liquid chromatography/mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (MS-MS). Major anthocyanins were identified as 3-O-glucosides cyanidin derivatives and minor peaks were identified as pelargonidin derivatives. Springer Science+Business Media B.V., 2007 Anthocyanins nationallicence Expression patterns nationallicence HPLC-ESI-MS nationallicence Saussurea medusa nationallicence ORF : open reading frame nationallicence RT-PCR : reverse transcription polymerase chain reaction nationallicence UTR : untranslated region nationallicence HPLC-ESI-MS : high performance liquid chromatography-electrospray ionization-mass spectrometry nationallicence HPLC : high performance liquid chromatography nationallicence BA : benzyladenine nationallicence NAA : naphthaleneacetic acid nationallicence Cheng Liqin Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut Xu Yanjun College of Science, China Agricultural University, 2 Yuanmingyuan Xi Lu, Haidian, 100094, Beijing, China aut Grotewold Erich Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center, The Ohio State University, 206 Rightmire Hall, 1060 Carmack road, 43210, Columbus, OH, USA aut Jin Zhiping Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut Wu Fengyan College of Science, China Agricultural University, 2 Yuanmingyuan Xi Lu, Haidian, 100094, Beijing, China aut Fu Chunxiang Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut Zhao Dexiu Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 63-73 0167-6857 89:1<63 2007 89 11240 https://doi.org/10.1007/s11240-007-9211-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9211-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Liqin Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xu Yanjun College of Science, China Agricultural University, 2 Yuanmingyuan Xi Lu, Haidian, 100094, Beijing, China aut NATIONALLICENCE 700 1- Grotewold Erich Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center, The Ohio State University, 206 Rightmire Hall, 1060 Carmack road, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Jin Zhiping Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wu Fengyan College of Science, China Agricultural University, 2 Yuanmingyuan Xi Lu, Haidian, 100094, Beijing, China aut NATIONALLICENCE 700 1- Fu Chunxiang Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Dexiu Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academic of Sciences, 20 Nanxin Cun, Xiangshan, Haidian, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 63-73 0167-6857 89:1<63 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer