caa a22 4500 463236319 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9203-x doi (NATIONALLICENCE)springer-10.1007/s11240-007-9203-x Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones [Elektronische Daten] [Sonja Gadzovska, Stéphane Maury, Alain Delaunay, Mirko Spasenoski, Claude Joseph, Daniel Hagège] Hypericum perforatum L. cell suspensions were evaluated for their viability, growth, dark gland formation and ability to produce phenylpropanoids and naphtodiantrones after elicitation with different jasmonic acid (JA) concentrations. Phenolic compounds were analyzed by high performance liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry (ESI-MS). The activities of two key enzymes of the phenylpropanoid/flavonoid pathways, phenylalanine ammonia lyase (PAL) and chalcone isomerase (CHI) were also monitored to estimate general channeling in the different metabolic pathways. A 6-fold increase of phenolic compounds, flavanols and flavonols after JA elicitation was observed in cells. In contrast, anthocyanins were in lower amounts in JA treated cells suggesting a modification of the channeling in the phenylpropanoid pathway. Similar accumulations with maxima after 4days of elicitation were found for naphtodianthrones (2.4-fold) such as hypericin and pseudohypericin in cells. At least a 6-8-fold increase of PAL and CHI activities was observed in JA elicited cells confirming a strong activation of the phenylpropanoid pathway. JA elicitation increased production of phenylpropanoids and naphtodianthrones in H. perforatum cell suspension without differentiation of dark glands under 16h photoperiod. Springer Science+Business Media B.V., 2007 Cell viability nationallicence Differentiation nationallicence Hypericin nationallicence Phenolic compounds nationallicence Pseudohypericin nationallicence CHI : Chalcone isomerase nationallicence ESI-MS : Electrospray ionisation-mass spectrometry nationallicence HPLC : High-performance liquid chromatography nationallicence JA : Jasmonic acid nationallicence [M-H]− : Negative molecular ion nationallicence MS2 : Collision fragment ions nationallicence PAL : Phenylalanine ammonia lyase nationallicence Gadzovska Sonja Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut Maury Stéphane Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut Delaunay Alain Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut Spasenoski Mirko Institute of Biology, Faculty of Natural Sciences and Mathematics, P.O. Box 162, 1000, Skopje, Macedonia aut Joseph Claude Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut Hagège Daniel Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 1-13 0167-6857 89:1<1 2007 89 11240 https://doi.org/10.1007/s11240-007-9203-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9203-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gadzovska Sonja Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut NATIONALLICENCE 700 1- Maury Stéphane Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut NATIONALLICENCE 700 1- Delaunay Alain Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut NATIONALLICENCE 700 1- Spasenoski Mirko Institute of Biology, Faculty of Natural Sciences and Mathematics, P.O. Box 162, 1000, Skopje, Macedonia aut NATIONALLICENCE 700 1- Joseph Claude Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut NATIONALLICENCE 700 1- Hagège Daniel Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté des Sciences, Université d'Orléans, rue de Chartres, P.O. Box 6759, 45067, Orléans, Cedex 2, France aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 1-13 0167-6857 89:1<1 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer