caa a22 4500 463236327 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9216-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9216-5 Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures [Elektronische Daten] [Neusa Steiner, Claudete Santa-Catarina, Vanildo Silveira, Eny Floh, Miguel Guerra] The aim of the present work was to determine the effects of exogenous polyamines (PAs) supplemented into a BM medium free of plant growth regulators (PGR), on the endogenous PAs, IAA and ABA contents and growth of A. angustifolia embryogenic cultures. The addition of PAs to the PGR-free BM medium, enhanced the growth of embryogenic cultures. PAs endogenous metabolism was significantly affected by exogenous PAs. PAs supplemented to a PGR-free BM medium, mainly Put and Spm, increased the endogenous IAA and ABA levels, showing a direct relationship -between PAs levels and ABA accumulation. These results direct new strategies to further improve A. angustifolia somatic embryogenesis, especially during the maturation phase, by culture medium manipulation using exogenous PAs. Springer Science+Business Media B.V., 2007 Conifer nationallicence Araucaria nationallicence Somatic embryogenesis nationallicence Putrescine nationallicence Spermidine nationallicence Spermine nationallicence BM : Culture medium of Gupta and Pullman (1991) nationallicence FM : Fresh mass nationallicence PAs : Polyamines nationallicence PGR : Plant growth regulators nationallicence Put : Putrescine nationallicence Spd : Spermidine nationallicence Spm : Spermine nationallicence Steiner Neusa Plant Developmental Physiology and Genetic Laboratory, Department of Plant Science, Federal University of Santa Catarina, CP 476, 88034-001, Florianopolis, Santa Catarina, Brazil aut Santa-Catarina Claudete Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut Silveira Vanildo Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut Floh Eny Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut Guerra Miguel Plant Developmental Physiology and Genetic Laboratory, Department of Plant Science, Federal University of Santa Catarina, CP 476, 88034-001, Florianopolis, Santa Catarina, Brazil aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 55-62 0167-6857 89:1<55 2007 89 11240 https://doi.org/10.1007/s11240-007-9216-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9216-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steiner Neusa Plant Developmental Physiology and Genetic Laboratory, Department of Plant Science, Federal University of Santa Catarina, CP 476, 88034-001, Florianopolis, Santa Catarina, Brazil aut NATIONALLICENCE 700 1- Santa-Catarina Claudete Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Silveira Vanildo Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Floh Eny Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Guerra Miguel Plant Developmental Physiology and Genetic Laboratory, Department of Plant Science, Federal University of Santa Catarina, CP 476, 88034-001, Florianopolis, Santa Catarina, Brazil aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 55-62 0167-6857 89:1<55 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer