caa a22 4500 463236335 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9215-6 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9215-6 On the effect of light on shoot regeneration in petunia [Elektronische Daten] [Moshe Reuveni, Dalia Evenor] Light has an important role in morphogenesis of cultured plant tissue. Morphogenesis depends on both photoperiod and spectrum. Thus, the effect of light on regeneration of shoots from several petunia species leaves was studied. Sterilized leaves of petunia were placed on MS medium supplemented with 5.7μM IAA and 2.25μM Zeatin. Leaf tissue was exposed to varying durations of light or darkness and regeneration was recorded. Petunia hybrida cultivar R did not regenerate in darkness while P. axillaris did regenerate in darkness. P. hybrida and P. axillaris regenerated in light. P. hybrida leaves on MS regeneration medium that were exposed to light, needed a minimum of 6days of irradiation to induced shoot regeneration. Dark-developing shoots were etiolated, which indicate that light was needed for induction of shoot morphogenesis. Crosses between P. hybrida and P. axillaris showed that there is a single dominant locus that is needed for regeneration in darkness and two loci for regeneration in light. Springer Science+Business Media B.V., 2007 Regeneration nationallicence Petunia nationallicence Light depedent nationallicence Zeatin : trans-6-(4-Hydroxy-3-methylbut-2-enylamino)purine nationallicence IAA : Indol-3-acetic acid nationallicence Reuveni Moshe Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, 50250, Bet Dagan, Israel aut Evenor Dalia Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, 50250, Bet Dagan, Israel aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 49-54 0167-6857 89:1<49 2007 89 11240 https://doi.org/10.1007/s11240-007-9215-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9215-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reuveni Moshe Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Evenor Dalia Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 49-54 0167-6857 89:1<49 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer