caa a22 4500 463236343 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s11240-007-9213-8 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9213-8 Improving freezing tolerance of transgenic tobacco expressing sucrose: sucrose 1-fructosyltransferase gene from Lactuca sativa [Elektronische Daten] [Hui-Juan Li, Ai-Fang Yang, Xue-Cheng Zhang, Feng Gao, Ju-Ren Zhang] Sucrose: sucrose 1-fructosyltransferase (1-SST) cDNA from Lactuca sativa, coding the enzyme responsible for lower degree polymers fructan biosynthesis, was cloned by RT-PCR and RACE methods. The 1-SST cDNA under the control of CaMV 35S promoter was introduced into tobacco by Agrobacterium-mediated leaf disc transformation protocol. Fructan synthesis in vitro and carbohydrate analysis showed that sense transgenic tobacco plant displayed sucrose: sucrose 1-fructosyltransferse activity. After freezing stress, significant increases in electrolyte leakage and malondialdehyde were found in the wild type and anti-sense transgenic plants, while no apparent differences were observed in sense transgenic plants. Meanwhile, water soluble carbohydrate, fructan and fructose of sense transgenic plants remarkably increased, compared with those of wild type and anti-sense plants. No significant difference was detected in superoxide dismutase activity between transgenic and wild type plants. The above results demonstrated that the expression of 1-SST gene improved the freezing resistance of transgenic tobacco plants. Springer Science+Business Media B.V., 2007 Freezing tolerance nationallicence Lactuca sativa nationallicence Ls 1- SST nationallicence RACE nationallicence Transgenic tobacco nationallicence CTAB : Cetyltrimethyl ammonium bromide nationallicence DP : Degree polymers nationallicence 1-FFT : Fructan: fructan 1-fructosyltransferase nationallicence MDA : Malondialdehyde nationallicence ORF : Open reading frame nationallicence RACE : Rapid amplification of cDNA ends nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence 1-SST : Sucrose: sucrose 1-fructosyltransferase nationallicence TLC : Thin layer chromatography nationallicence WSC : Water soluble carbohydrate nationallicence Li Hui-Juan College of Life Science and Technology, Ocean University of China, 266003, Qingdao, People's Republic of China aut Yang Ai-Fang School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Zhang Xue-Cheng College of Life Science and Technology, Ocean University of China, 266003, Qingdao, People's Republic of China aut Gao Feng School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Zhang Ju-Ren School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 37-48 0167-6857 89:1<37 2007 89 11240 https://doi.org/10.1007/s11240-007-9213-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9213-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Hui-Juan College of Life Science and Technology, Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Yang Ai-Fang School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xue-Cheng College of Life Science and Technology, Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Gao Feng School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ju-Ren School of Life Science, Shandong University, 27 Shanda South Road, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/1(2007-04-01), 37-48 0167-6857 89:1<37 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer