caa a22 4500 463236351 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9237-0 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9237-0 Exogenous trehalose affects morphogenesis invitro of jojoba [Elektronische Daten] [Berta Llorente, Leonardo Juarez, Nancy Apóstolo] Trehalose is a stress protecting and reserve carbohydrate in a variety of organisms. The effect of trehalose on micropropagation of jojoba [Simmondsia chinensis (Link) Schneider] was investigated using a modified Murashige-Skoog as the basal medium (BM). Proliferation rate was significantly higher in explants grown on BM+1mM trehalose compared to that in the phytohormone control medium. In multiplication stage, shoot proliferation rate of 4.8 was achieved in BM with 4.44μM BA and 1mM trehalose. The rooting induction with 14.7μM IBA had a significant effect on root regeneration; the highest rooting percentage (46.6%) was obtained with 7days of induction. The incorporation of trehalose to the IBA-rhizogenesis media decreased basal callus but with trehalose alone, root development was scant. In vitro plants showed anatomical features typical for the acclimatization stage. Springer Science+Business Media B.V., 2007 Micropropagation nationallicence Oligosaccharide nationallicence Plant growth regulators nationallicence Plant tissue culture nationallicence Simmondsia chinensis nationallicence BA : N6-benzyladenine nationallicence BM : Basic medium nationallicence GA3 : Gibberellic acid A3 nationallicence IBA : Indole-3-butyric acid nationallicence MS : Murashige-Skoog salts nationallicence RM : Rooting medium nationallicence Llorente Berta Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Lujan, Buenos Aires, Argentina aut Juarez Leonardo Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Lujan, Buenos Aires, Argentina aut Apóstolo Nancy Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Lujan, Buenos Aires, Argentina aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 193-201 0167-6857 89:2-3<193 2007 89 11240 https://doi.org/10.1007/s11240-007-9237-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9237-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Llorente Berta Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Lujan, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Juarez Leonardo Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Lujan, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Apóstolo Nancy Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Lujan, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 193-201 0167-6857 89:2-3<193 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer