caa a22 4500 46323636X CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9230-7 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9230-7 Efficient in vitro regeneration systems for Vaccinium species [Elektronische Daten] [Julia Meiners, Melanie Schwab, Iris Szankowski] Efficient protocols for shoot regeneration from leaf explants suitable for micropropagation as well as for the development of transgenic plants were developed for blueberry (Vaccinium corymbosum) and lingonberry (Vaccinium vitis-idaea) cultivars. Nodal segments were used to initiate in vitro shoot cultures of lingonberry cultivar ‘Red Pearl' and southern highbush blueberry cultivar ‘Ozarkblue'. In order to develop an optimized regeneration procedure, different types and concentrations of plant growth regulators were tested to induce adventitious shoot regeneration on excised leaves from micropropagated shoots of both cultivars. The effect on percentage regeneration and number of shoots per explant was investigated. Results indicated that zeatin was superior to TDZ and meta-topolin in promoting adventitious shoot formation. A concentration of 20μM zeatin was most effective in promoting shoot regeneration in both cultivars, in case of ‘Red Pearl' along with 1μM NAA. Shoots were either allowed to root in vitro on medium containing IBA or NAA or ex vitro in a fog tunnel. IBA was superior to NAA for induction of root development in vitro in both Vaccinium cultivars. Ex vitro rooting under high humidity was tested with cuttings from mature field-grown plants, from acclimatized tissue culture derived plants and with unrooted in vitro proliferated shoots planted directly. It was found that in vitro shoots rooted better under fog than cuttings from the other plant sources and rooting was equivalent to that achieved in vitro. Springer Science+Business Media B.V., 2007 Blueberry nationallicence Lingonberry nationallicence TDZ nationallicence Meta-topolin nationallicence Zeatin nationallicence IBA : indole-3-butyric acid nationallicence NAA : naphtaleneacetic acid nationallicence TDZ : thidiazuron nationallicence Meiners Julia Institute of Biological Production Systems, Fruit Science Section, Gottfried Wilhelm Leibniz University of Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany aut Schwab Melanie Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany aut Szankowski Iris Institute of Biological Production Systems, Fruit Science Section, Gottfried Wilhelm Leibniz University of Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 169-176 0167-6857 89:2-3<169 2007 89 11240 https://doi.org/10.1007/s11240-007-9230-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9230-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meiners Julia Institute of Biological Production Systems, Fruit Science Section, Gottfried Wilhelm Leibniz University of Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany aut NATIONALLICENCE 700 1- Schwab Melanie Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany aut NATIONALLICENCE 700 1- Szankowski Iris Institute of Biological Production Systems, Fruit Science Section, Gottfried Wilhelm Leibniz University of Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 169-176 0167-6857 89:2-3<169 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer