caa a22 4500 463236386 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9245-0 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9245-0 Production and characterization of asymmetric hybrids between upland cotton Coker 201 ( Gossypium hirsutum ) and wild cotton ( G. klozschianum Anderss) [Elektronische Daten] [Xi-yan Yang, Xian-long Zhang, Shuang-xia Jin, Li-li Fu, Ling-gang Wang] Asymmetric somatic hybrids were obtained between Gossypium hirsutum Coker 201 and wild cotton G. klozschianum Anderss. An investigation on the effect of ultraviolet (UV) irradiation on donor protoplasts was carried out, and the lethal dose was determined to be 38.7Jcm−2. We firstly screened the putative hybrids by the color of the calli produced, followed by morphological, cytological, and molecular analysis of putative hybrid plants. Most regenerated plants derived from fused protoplasts displayed a recipient-like morphology, while some showed an intermediate phenotype between Coker 201 and G. klozschianum. Chromosome numbers in these somatic hybrids ranged from 54 to 74. The hybrids were verified by random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR). Absence or co-existence of parents' genome DNA fragments was identified through molecular analysis. The heredity of cytoplasm was investigated by cleaved amplified polymorphic sequence (CAPS) analysis using mitochondrial and chloroplast universal primer pairs. The results indicated that recombination and rearrangements might have occurred in some regions of mitochondria (mt) and chloroplast (cp) DNA. To our knowledge, this is the first report about asymmetric protoplast fusion in cotton, and the hybrids obtained would be useful for breeding programs. Springer Science+Business Media B.V., 2007 Asymmetric somatic hybrids nationallicence Cotton nationallicence Protoplast fusion nationallicence UV irradiation nationallicence CAPS : Cleaved amplified polymorphic sequence nationallicence cpDNA : Chloroplast DNA nationallicence CPW : Cell and protoplast washing solution (Frearson etal. 1973) nationallicence CTAB : Cetyltrimethyl ammoniumbromide nationallicence FDA : Fluorescein diacetate nationallicence GFP : Green fluorescent protein nationallicence IBA : Indole-3-butyric acid nationallicence IOA : Iodoacetamide nationallicence MES : 2, (N-morpholino) ethane sulfonic acid nationallicence MSB : MS medium (Murashige and Skoog 1962) and B5 (Gamborg etal. 1968) vitamins nationallicence mtDNA : Mitochondrial DNA nationallicence PAGE : Polyacrylamide gel electrophoresis nationallicence RAPD : Random amplified polymorphic DNA nationallicence SSR : Simple sequence repeat nationallicence UV : Ultraviolet nationallicence Yang Xi-yan National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Zhang Xian-long National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Jin Shuang-xia National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Fu Li-li National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Wang Ling-gang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 225-235 0167-6857 89:2-3<225 2007 89 11240 https://doi.org/10.1007/s11240-007-9245-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9245-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xi-yan National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Zhang Xian-long National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Jin Shuang-xia National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Fu Li-li National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Wang Ling-gang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 225-235 0167-6857 89:2-3<225 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer