caa a22 4500 463236394 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9222-7 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9222-7 A brief temperature pulse enhances the competency of microspores for androgenesis in Datura metel [Elektronische Daten] [Mohamed Iqbal, Kolitha Wijesekara] Androgenesis may be induced in plants by a stress application on microspores or anthers. Temperature stress treatments have generally been confined to a single temperature regime (above or below ambient) lasting from a few hours to days. We introduced a gradient with two temperature pulses (30s each) in the stress application on anthers of Datura metel L. by stepping the temperature up and down for a total period of 60s. Anthers were immersed in sterile water preheated and cooled to the desired temperature and cultured on Nitsch medium. The temperature pulse gradient significantly improved androgenesis compared to single temperature treatments, resulting in increased mean embryogenesis of 128% over control for 45°/15°C, 110% for 45°/10°C, 113% for 40°/10°C and 96% for 45°/5°C. The 45°/10°C gradient also significantly increased the number of dividing microspores observed, after 14days of anther culture. Besides the differential of the gradient, the temperature limit was important, with anthers not tolerating temperatures beyond 45°C. The temperature pulse gradient applied at an early stage of culture may increase the window of competency of microspores for androgenesis. Springer Science+Business Media B.V., 2007 Anther culture nationallicence Androgenesis nationallicence Haploids nationallicence Embryo conversion nationallicence Temperature stress nationallicence Iqbal Mohamed Plant Reproductive Biology, Institute of Fundamental Studies, Hantane Road, Kandy, Sri Lanka aut Wijesekara Kolitha Plant Reproductive Biology, Institute of Fundamental Studies, Hantane Road, Kandy, Sri Lanka aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 141-149 0167-6857 89:2-3<141 2007 89 11240 https://doi.org/10.1007/s11240-007-9222-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9222-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iqbal Mohamed Plant Reproductive Biology, Institute of Fundamental Studies, Hantane Road, Kandy, Sri Lanka aut NATIONALLICENCE 700 1- Wijesekara Kolitha Plant Reproductive Biology, Institute of Fundamental Studies, Hantane Road, Kandy, Sri Lanka aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 141-149 0167-6857 89:2-3<141 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer