caa a22 4500 463236424 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9214-7 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9214-7 Indirect shoot organogenesis from leaves of Dieffenbachia cv. Camouflage [Elektronische Daten] [Xiuli Shen, Jianjun Chen, Michael Kane] A novel protocol for indirect shoot organogenesis of Dieffenbachia cv. Camouflage was established using leaf explants excised from in vitro shoot cultures. The frequency of callus formation reached 96% for explants cultured on Murashige and Skoog (1962) basal medium supplemented with 5μM thidiazuron and 1μM 2,4-dichlorophenozyacetic acid. The number of shoots regenerated was high, with up to 7.9 shoots produced per callus cultured on basal medium supplemented with 40μM N 6-(Δ2-isopentenyl)adenine and 2μM indole-3-acetic acid. Regenerated shoots rooted well in a soilless substrate, acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse conditions. Somaclonal variations in leaf variegation, color, and morphology have been observed in regenerated plants. Springer Science+Business Media B.V., 2007 Dieffenbachia nationallicence Ornamental foliage plants nationallicence Shoot organogenesis nationallicence 2,4-D : 2,4-Dichlorophenozyacetic acid nationallicence 2iP : N 6(Δ2-isopentenyl)adenine nationallicence BA : 6-benzyladenine nationallicence CPPU : N(2-chloro-4-pyridyl)-N-phenylurea nationallicence IAA : indole-3-acetic acid nationallicence NAA : 1-naphthalene acetic acid nationallicence TDZ : thidiazuron nationallicence Shen Xiuli Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-067, Gainesville, FL, USA aut Chen Jianjun Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-067, Gainesville, FL, USA aut Kane Michael Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-067, Gainesville, FL, USA aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 83-90 0167-6857 89:2-3<83 2007 89 11240 https://doi.org/10.1007/s11240-007-9214-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9214-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Xiuli Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-067, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Chen Jianjun Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-067, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Kane Michael Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-067, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 83-90 0167-6857 89:2-3<83 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer