caa a22 4500 463236440 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9217-4 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9217-4 Epigenetic variation in tissue cultured Gaura lindheimeri [Elektronische Daten] [Grace Pietsch, Neil Anderson] High levels of variability were observed in tissue cultured Gaura lindheimeri genotypes when flowered in situ. Tissue culture treatments for chromosome doubling (colchicine: 0, 0.25, 1.25mM; trifluralin: 0, 15, 30μM) were all highly variable for morphological traits. Experiment No. 1 tested the tissue culture protocols used. In the first control (C1), plantlets were subcultured continuously. Nodes were excised and placed on solid medium for the second control (C2). In the third control (C3), nodes were excised and put in liquid medium for 24h at room temperature. The fourth control (C4) was the same as the third control except liquid cultures were moved to 4°C for 48h after treatment at room temperature. Experiment No. 2 examined the stability of the variation. Representative plants with different traits were selected for clonal propagation and grown in a replicated trial in the greenhouse. Several morphological traits (flower size, leaf length:width ratios, petal length:width ratios, and flower color) were measured. All of the controls had as much variability as the treated plants. Flower size of the first flower for plant number 01G-02 was significantly different in C1 compared with C3, but not with C2 and C4. Plant number 443-1 (white) was more stable in the replicated trial for the flower size than plant number 01G-02 (pink). All traits measured for plant number 01G-02 were unstable; most flower colors and patterns reverted back to the original color of the non-tissue cultured plants. The somaclonal variation observed was epigenetic in nature, indicating changes in DNA methylation. Springer Science+Business Media B.V., 2007 Flower pigmentation nationallicence Length:width ratios nationallicence Somaclonal variation nationallicence Tissue culture nationallicence l:w : Length:width nationallicence Pietsch Grace Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave, 55108, St. Paul, MN, USA aut Anderson Neil Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave, 55108, St. Paul, MN, USA aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 91-103 0167-6857 89:2-3<91 2007 89 11240 https://doi.org/10.1007/s11240-007-9217-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9217-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pietsch Grace Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Anderson Neil Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave, 55108, St. Paul, MN, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 91-103 0167-6857 89:2-3<91 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer