caa a22 4500 463236459 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9219-2 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9219-2 Somatic embryogenesis and plant regeneration of litchi ( Litchi chinensis Sonn.) from leaves of mature phase trees [Elektronische Daten] [Simon Raharjo, Richard Litz] Embryogenic cultures were induced from leaflets from new vegetative flushes of mature ‘Brewster' litchi trees on B5 medium containing 400mgl−1 glutamine, 200mg l−1 casein hydrolysate, 30gl−1 sucrose, 4.52μM 2,4-D, 9.30μM kinetin and 3gl−1 gellan gum in darkness. Embryogenic cultures consisting of proembryonic cells and masses were maintained either on semi-solid MS medium supplemented with 4.52μM 2,4-D and 0.91μM zeatin or as embryogenic suspension cultures in liquid medium of the same composition. Maturation of somatic embryos occurred on semi-solid MS medium with 5-20% (v/v) filter-sterilized coconut water in darkness. Recovery of plants from somatic embryos was improved with 14.4μM GA3 on half-strength MS medium with 0.2gl−1 activated charcoal under a 16h photoperiod provided by cool white fluorescent lights (60-80μmols−1m−2). Plants have been successfully acclimatized in the greenhouse. Springer Science+Business Media B.V., 2007 Lychee nationallicence Somatic embryo nationallicence Suspension culture nationallicence BA : Benzylaminopurine nationallicence B5 : Gamborg etal. (1968) nationallicence CW : Coconut water nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence GA3 : Gibberellic acid nationallicence MS : Murashige and Skoog (1962) nationallicence PEM : Proembryonal mass nationallicence Raharjo Simon Tropical Research and Education Center, University of Florida, 18905 S.W. 280 Street, 33031-3314, Homestead, FL, USA aut Litz Richard Tropical Research and Education Center, University of Florida, 18905 S.W. 280 Street, 33031-3314, Homestead, FL, USA aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 113-119 0167-6857 89:2-3<113 2007 89 11240 https://doi.org/10.1007/s11240-007-9219-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9219-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raharjo Simon Tropical Research and Education Center, University of Florida, 18905 S.W. 280 Street, 33031-3314, Homestead, FL, USA aut NATIONALLICENCE 700 1- Litz Richard Tropical Research and Education Center, University of Florida, 18905 S.W. 280 Street, 33031-3314, Homestead, FL, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 113-119 0167-6857 89:2-3<113 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer