caa a22 4500 463236467 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9239-y doi (NATIONALLICENCE)springer-10.1007/s11240-007-9239-y The use of excised roots from invitro culture for the determination of superoxide production in plants [Elektronische Daten] [E. Cosi, P. Bettini, D. Bindi, M. Buiatti] The production of reactive oxygen species (ROS) in plants is a common event in metabolic and physiological processes as well as in the response to biotic and abiotic stress. In this paper we will report that root tissue from axenically grown tomato cultivars and Lycopersicon wild species can be used for the determination of superoxide production. Superoxide generation was evaluated following the treatment of root tissues with two general elicitors of the defence response: laminarin and calcium ionophore A23187. Results demonstrated that elicitor reactivity in terms of superoxide generation of the tomato cultivars and the wild species used was different. This suggested varying levels of competence for non-specific active defence. The proposed technique merges the advantages of invitro cultures and of whole tissues and also demonstrates that root tissue is a suitable material for evaluating free radical release. Springer Science+Business Media B.V., 2007 Biotic stress nationallicence Lycopersicon nationallicence Reactive oxygen species nationallicence Tissue culture nationallicence ANOVA : Analysis of variance nationallicence CAT : Catalase nationallicence Cyt c : Cytochromec nationallicence HPRGs : Hydroxyproline-rich glycoproteins nationallicence HR : Hypersensitive response nationallicence LS : Linsmajer and Skoog medium nationallicence NADPH : Nicotinamide adenine dinucleoside phosphate nationallicence ROI : Reactive oxygen intermediates nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence Cosi E. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut Bettini P. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut Bindi D. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut Buiatti M. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 203-209 0167-6857 89:2-3<203 2007 89 11240 https://doi.org/10.1007/s11240-007-9239-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9239-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cosi E. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut NATIONALLICENCE 700 1- Bettini P. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut NATIONALLICENCE 700 1- Bindi D. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut NATIONALLICENCE 700 1- Buiatti M. Dipartimento di Biologia animale e Genetica ‘Leo Pardi', Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 203-209 0167-6857 89:2-3<203 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer