caa a22 4500 463236475 CHVBK 20180405153258.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9248-x doi (NATIONALLICENCE)springer-10.1007/s11240-007-9248-x A single genetic locus in chromosome 1 controls conditional browning during the induction of calli from mature seeds of Oryza sativa ssp. indica [Elektronische Daten] [Zhong Li, Shihua Duan, Jin Kong, Shaoqing Li, Yangsheng Li, Yingguo Zhu] Being the crucial step for rice transgenic manipulation, callus culture from mature seeds is severely restricted by browning of induced calli, especially in the case of indica (Oryza Sativa L.) rice. Once this browning occurs, the callus will die and no embryonic calli can be obtained for regeneration. Here we report an induction procedure that overcomes callus browning was found. To clarify the inheritance pattern of callus browning, two reciprocal crosses F2 and two backcrosses BC1 were made between indica cultivar inbred lines 93-11 and YueTaiB (YTB) which produced normal and browning respectively in the same induction medium. The ratio of browning to normal in the reciprocal F2 and backcross (BC1) populations tested was approximately 1:3 and 1:1, respectively, these results indicate that callus browning is controlled by one single chromosomal locus which is tentatively named Ic1 (Induced callus 1). The genetic mapping of this locus was carried out using microsatellite markers (SSR) in a 216 extremely browning F2 seed callus. The analysis of genetic linkage indicated that one single locus that mapped to chromosome 1 was correlated to callus browning, and the closest marker in this study was mapped within 1.9cM from the target locus. Springer Science+Business Media B.V., 2007 Callus browning nationallicence Inheritance pattern nationallicence Genetic map nationallicence Microsatellite markers nationallicence Li Zhong Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Duan Shihua Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Kong Jin Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Li Shaoqing Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Li Yangsheng Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Zhu Yingguo Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 237-245 0167-6857 89:2-3<237 2007 89 11240 https://doi.org/10.1007/s11240-007-9248-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9248-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Zhong Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Duan Shihua Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Kong Jin Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Li Shaoqing Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Li Yangsheng Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhu Yingguo Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 237-245 0167-6857 89:2-3<237 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer