caa a22 4500 463236483 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9240-5 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9240-5 Obtaining autotetraploids invitro at a high frequency in Citrus sinensis [Elektronische Daten] [June Zhang, Min Zhang, Xiuxin Deng] Tetraploid plants are essential for interploid hybridization to create triploid seedless citrus. Here we report a simple and efficient invitro method for generating autotetraploids for sweet orange (Citrus sinensis). Cell-division activity in ‘Anliucheng' sweet orange callus was analyzed using flow cytometry to determine the peak frequency of cell division at which time callus in a liquid media and solid media was treated with 1000mgl−1 colchicine. The percentage of the DNA-content-varied cells in the callus increased markedly from 11.0% to 44.4% and to 59.0% for liquid and solid media respectively. A total of 20 tetraploid plantlets were recovered via embryogenesis from 47 plantlets regenerated from the treated callus. All the autotetraploids were derived from different embryoids. Autotetraploids will be useful parents for interploid hybridization to generate commercially valuable seedless triploid citrus cultivars. Springer Science+Business Media B.V., 2007 Autotetraploid nationallicence Callus nationallicence Citrus sinensis nationallicence Colchicine nationallicence Flow cytometry nationallicence BA : 6-Benzylaminopurine nationallicence KT : Kinetin nationallicence MT : Murashige and Tucker (1969) nationallicence NAA : α−Naphthalene acetic acid nationallicence Zhang June National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China aut Zhang Min National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China aut Deng Xiuxin National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 211-216 0167-6857 89:2-3<211 2007 89 11240 https://doi.org/10.1007/s11240-007-9240-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9240-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang June National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhang Min National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Deng Xiuxin National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 211-216 0167-6857 89:2-3<211 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer