caa a22 4500 463236491 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9231-6 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9231-6 Overexpression of a NTR1 in transgenic soybean confers tolerance to water stress [Elektronische Daten] [Ren-Gao Xue, Biao Zhang, Hong-Feng Xie] Methyl jasmonate (MeJA) is an important plant regulator that involves in plant development and regulates the expression of plant defense genes in response to various stresses such as wounding, drought, and pathogens. In order to determine the physiological role of endogenous MeJA in plants, a NTR1 from Brassica campestris encoding a jasmonic acid carboxyl methyltransferase that produces methyl jasmonate was constructed under the control of CaMV 35S promoter and transformed into soybean [Glycine max (L) Merrill]. The transgenic soybean plants constitutively expressed the NTR1 and accumulated more MeJA levels than wild type plants. Overexpression of the gene in transgenic soybean conferred tolerance to dehydration during seed germination and seedling growth as reflected by the percentage of the fresh weight of seedlings. In addition, the transgenic soybean plants also conferred better capacity to retain water than wild type plants when drought tolerance was tested using detached leaves. Springer Science+Business Media B.V., 2007 Drought tolerance nationallicence Methyl jasmonate nationallicence NTR1 nationallicence Soybean nationallicence Water stress nationallicence AS : Acetosyringone nationallicence BAP : 6-benzylaminopurine nationallicence JA : Jasmonic acid nationallicence MeJA : Methyl jasmonate nationallicence POD : Peroxidase nationallicence PPT : Phosphinothricin nationallicence SOD : Superoxide dismutase nationallicence VSPs : Vegetative storage proteins nationallicence Xue Ren-Gao Department of Life & Science, Laiyang Agricultural College, 266109, Qingdao, China aut Zhang Biao Department of Life & Science, Laiyang Agricultural College, 266109, Qingdao, China aut Xie Hong-Feng Department of Life & Science, Laiyang Agricultural College, 266109, Qingdao, China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 177-183 0167-6857 89:2-3<177 2007 89 11240 https://doi.org/10.1007/s11240-007-9231-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9231-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xue Ren-Gao Department of Life & Science, Laiyang Agricultural College, 266109, Qingdao, China aut NATIONALLICENCE 700 1- Zhang Biao Department of Life & Science, Laiyang Agricultural College, 266109, Qingdao, China aut NATIONALLICENCE 700 1- Xie Hong-Feng Department of Life & Science, Laiyang Agricultural College, 266109, Qingdao, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 177-183 0167-6857 89:2-3<177 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer