caa a22 4500 463236513 CHVBK 20180405153259.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s11240-007-9223-6 doi (NATIONALLICENCE)springer-10.1007/s11240-007-9223-6 Plant regeneration from embryogenic suspension-derived protoplasts of ginger ( Zingiber officinale Rosc.) [Elektronische Daten] [Yinghua Guo, Jinhe Bai, Zhenxian Zhang] A procedure is described to regenerate plants from embryogenic suspension-derived protoplasts of ginger (Zingiber officinale Rosc.). Somatic embryogenic calli were induced from ginger shoot tips on solid MS medium with half the concentration of NH4NO3 and supplemented with 1.0mgl−1 2,4-Dichloroacetic acid (2, 4-D) and 0.2mgl−1Kin. Rapid-growing and well-dispersed suspension cultures were established by subculturing the embryogenic calli in the same liquid medium. Protoplasts were isolated from embryogenic suspensions with an enzyme solution composed of 4.0mgl−1 cellulase, 1.0mg l−1 macerozyme, 0.1mgl−1 pectolyase, 11% mannitol, 0.5% CaCl2 and 0.1% 2-(N-morpholino) ethane sulphonic acid (MES) for 12-14h at 27°C with a yield of 6.27×106 protoplastsg−1 fresh weight. The protoplasts were cultured initially in liquid MS medium with 1.0mgl−1 2, 4-D and 0.2mgl−1Kin. Then the protoplast-derived calli (1.5cm2) were transferred to a basal MS medium containing 0.2mgl−1 2, 4-D, 5.0mgl−1 benzyladenine (BA), 3% sucrose and 0.7% agar. The white somatic embryos were transferred to MS medium lacking growth regulators for shoot development. Shoots developed into complete plantlets on a solid MS medium supplemented with 2.0mgl−1 BA and 0.6mgl−1 α-Naphthaleneacetic acid (NAA). In addition, the effects of AgNO3, activated charcoal (AC) and ascorbic acid (AA) on browning of protoplast-derived calli are discussed. Springer Science+Business Media B.V., 2007 Ginger nationallicence Protoplast nationallicence Embryogenic suspensions nationallicence Plant regeneration nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence AC : Activated charcoal nationallicence AA : Ascorbic acid nationallicence BA : Benzyladenine nationallicence CH : Casein hydrolysate nationallicence DTT : Citric acid and 1,4-dithio-dl-threitol nationallicence FDA : Fluorescein diacetate nationallicence Kin : 6-Furfurylaminopurine nationallicence MES : 2-(N-morpholino) ethane sulphonic acid nationallicence MS : Murashige and Skoog medium (Murashige and Skoog 1962) nationallicence NAA : α-Naphthaleneacetic acid nationallicence PVP : Polyvinylpyrrolidone nationallicence Guo Yinghua Agronomy and Biotechnology College, China Agriculture University, 100094, Beijing, China aut Bai Jinhe Mid-Columbia Agricultural Research & Extension Center, Oregon State University, 7031-9512, Corvallis, USA aut Zhang Zhenxian Agronomy and Biotechnology College, China Agriculture University, 100094, Beijing, China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 151-157 0167-6857 89:2-3<151 2007 89 11240 https://doi.org/10.1007/s11240-007-9223-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9223-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo Yinghua Agronomy and Biotechnology College, China Agriculture University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Bai Jinhe Mid-Columbia Agricultural Research & Extension Center, Oregon State University, 7031-9512, Corvallis, USA aut NATIONALLICENCE 700 1- Zhang Zhenxian Agronomy and Biotechnology College, China Agriculture University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 89/2-3(2007-05-01), 151-157 0167-6857 89:2-3<151 2007 89 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer